RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells

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  • dc.contributor.author Martínez-Moro, Álvaro
  • dc.contributor.author González-Brusi, Leopoldo
  • dc.contributor.author Lamas-Toranzo, Ismael
  • dc.contributor.author O'Callaghan, Elena
  • dc.contributor.author Esteve-Codina, Anna
  • dc.contributor.author Lonergan, Pat
  • dc.contributor.author Bermejo-Álvarez, Pablo
  • dc.date.accessioned 2022-11-03T07:10:43Z
  • dc.date.available 2022-11-03T07:10:43Z
  • dc.date.issued 2022
  • dc.description.abstract Cumulus cells provide an interesting biological material to perform analyses to understand the molecular clues determining oocyte competence. The objective of this study was to analyze the transcriptional differences between cumulus cells from oocytes exhibiting different developmental potentials following individual in vitro embryo production by RNA-seq. Cumulus cells were allocated into three groups according to the developmental potential of the oocyte following fertilization: (1) oocytes developing to blastocysts (Bl+), (2) oocytes cleaving but arresting development before the blastocyst stage (Bl-), and (3) oocytes not cleaving (Cl-). RNAseq was performed on 4 (Cl-) or 5 samples (Bl+ and Bl-) of cumulus cells pooled from 10 cumulus-oocyte complexes per group. A total of 49, 50, and 18 differentially expressed genes (DEGs) were detected in the comparisons Bl+ versus Bl-, Bl+ versus Cl- and Bl- versus Cl-, respectively, showing a fold change greater than 1.5 at an adjusted p value <0.05. Focussing on DEGs in cumulus cells from Bl+ group, 10 DEGs were common to both comparisons (10/49 from Bl+ vs. Bl-, 10/50 from Bl+ vs. Cl-). These DEGs correspond to 6 upregulated genes (HBE1, ITGA1, PAPPA, AKAP12, ITGA5, and SLC1A4), and 4 downregulated genes (GSTA1, PSMB8, FMOD, and SFRP4) in Bl+ compared to the other groups, from which 7 were validated by quantitative PCR (HBE1, ITGA1, PAPPA, AKAP12, ITGA5, PSMB8 and SFRP4). These genes are involved in critical biological functions such as integrin-mediated cell adhesion, oxygen availability, IGF and Wnt signaling or PKA pathway, highlighting specific biological processes altered in incompetent in vitro maturation oocytes.
  • dc.description.sponsorship This work was funded by the projects IND2017/BIO-7748 from Madrid Region Government and AGL2017-84908-R and PID2020-117501RB-I00 by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO). AMM was funded by project IND2017/BIO-7748 and ILT by a FPI fellowship by MINECO.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Martínez-Moro Á, González-Brusi L, Lamas-Toranzo I, O'Callaghan E, Esteve-Codina A, Lonergan P, Bermejo-Álvarez P. RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells. Mol Reprod Dev. 2022 Sep;89(9):399-412. DOI: 10.1002/mrd.23631
  • dc.identifier.doi http://dx.doi.org/10.1002/mrd.23631
  • dc.identifier.issn 1040-452X
  • dc.identifier.uri http://hdl.handle.net/10230/54667
  • dc.language.iso eng
  • dc.publisher Wiley
  • dc.relation.ispartof Mol Reprod Dev. 2022 Sep;89(9):399-412
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-84908-R
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-117501RB-I00
  • dc.rights © 2022 The Authors. Molecular Reproduction and Development published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
  • dc.subject.keyword Granulosa cell
  • dc.subject.keyword In vitro maturation
  • dc.subject.keyword Integrin
  • dc.subject.keyword Oocyte quality
  • dc.subject.keyword Transcription
  • dc.title RNA-sequencing reveals genes linked with oocyte developmental potential in bovine cumulus cells
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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