Detection of senescence markers during mammalian embryonic development

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• dc.contributor.author Storer, Mekayla, 1981-
• dc.contributor.author Keyes, William M., 1973-
• dc.date.accessioned 2019-02-28T10:44:32Z
• dc.date.available 2019-02-28T10:44:32Z
• dc.date.issued 2017
• dc.description.abstract Senescence-associated β-galactosidase (SAβ-gal) is a convenient histological technique used to identify senescent cells. Its ease of use is helpful to initially screen and detect senescent cells in heterogeneous cell populations both in vitro and in vivo. However, SAβ-gal staining is not an unequivocal marker of the senescent state, and diagnosis of such usually requires additional markers demonstrating an absence of proliferation and expression of cell-cycle inhibitors. Nonetheless, SAβ-gal remains one of the most widely used biomarkers of senescent cells. Recently, by measuring SAβ-gal activity, the expression of the cyclin-dependent kinase inhibitor p21 (waf1/cip1) and demonstrating a lack of proliferation, we identified senescent cells in the developing embryo. This chapter describes the methods for identifying cellular senescence in the embryo, detailing protocols for the detection of SAβ-gal activity in both sections and at the whole mount level, and immunohistochemistry protocols for the detection of additional biomarkers of senescence.
• dc.description.sponsorship This work was funded by Grants SAF2010-18829 and SAF2013-49082-P to W.M.K. from the Spanish Ministry for Economy and Competitiveness, the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR) from the Generalitat de Catalunya, and CRG core funding
• dc.format.mimetype application/pdf
• dc.identifier.citation Storer M, Keyes WM. Detection of senescence markers during mammalian embryonic development. Methods Mol Biol. 2017;1534:199-210. DOI: 10.1007/978-1-4939-6670-7_19
• dc.identifier.doi http://dx.doi.org/10.1007/978-1-4939-6670-7_19
• dc.identifier.issn 1064-3745
• dc.identifier.uri http://hdl.handle.net/10230/36705
• dc.language.iso eng
• dc.publisher Humana Press (Springer Imprint)
• dc.relation.ispartof Methods in Molecular Biology. 2017;1534:199-210
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2010-18829
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2013-49082-P
• dc.rights © Springer The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-6670-7_19
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.subject.other Apoptosi
• dc.subject.other Mamífers
• dc.subject.other Embriologia
• dc.subject.other Cèl·lules -- Envelliment
• dc.title Detection of senescence markers during mammalian embryonic development
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion