A coordinated response at the transcriptome and interactome level is required to ensure uropathogenic Escherichia coli survival during bacteremia

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• dc.contributor.author Sanchez de Groot, Natalia
• dc.contributor.author Torrent Burgas, Marc
• dc.date.accessioned 2019-10-25T11:12:03Z
• dc.date.available 2019-10-25T11:12:03Z
• dc.date.issued 2019
• dc.description.abstract Localized infections or disruption of the skin barrier can enable the entry of bacteria into the bloodstream, possibly leading to acute inflammation and sepsis. There is currently no holistic view on how bacteria can survive and spread in the bloodstream. In this context, we combined transposon mutagenesis, gene-expression profiling and a protein interaction network analysis to examine how uropathogenic Escherichia coli can proliferate in blood. Our results indicate that, upon migration from the urea to serum, E. coli reacts to the osmolarity difference, triggering a transcriptomic response in order to express survival genes. The proteins codified by these genes are precisely organized at the interactome level and specifically target short linear motifs located in disordered regions of host proteins. Such a coordinated response helps to explain how bacteria can adapt to and survive environmental changes within the host. Overall, our results provide a general framework for the study of bacteremia and reveal new targets for potential study as novel antimicrobials.
• dc.description.sponsorship This study was funded by the Ministerio de Economía y Competitividad (SAF2014-56568-R, SAF2015-72518-EXP and SAF2017-82158-R) and a Research Grant awarded in 2016 by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID), all of which was provided to MT. MT would like to acknowledge support from the Programa Ramón y Cajal (RYC-2012-09999). NSdG acknowledges the support of the Ministerio de Economía y Competitividad, the ‘Centro de Excelencia Severo Ochoa 2013-2017’ and the CERCA Programme from the Generalitat de Catalunya.
• dc.format.mimetype application/pdf
• dc.identifier.citation de Groot NS, Torrent Burgas M. A coordinated response at the transcriptome and interactome level is required to ensure uropathogenic Escherichia coli survival during bacteremia. Microorganisms. 2019; 7(9). pii: E292. DOI 10.3390/microorganisms7090292
• dc.identifier.doi http://dx.doi.org/10.3390/microorganisms7090292
• dc.identifier.issn 2076-2607
• dc.identifier.uri http://hdl.handle.net/10230/42510
• dc.language.iso eng
• dc.publisher MDPI
• dc.relation.ispartof Microorganisms. 2019; 7(9). pii: E292
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2014-56568-R
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2015-72518-EXP
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/SAF2017-82158-R
• dc.rights © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Escherichia coli
• dc.subject.keyword Antimicrobials
• dc.subject.keyword Bacteremia
• dc.subject.keyword Urinary tract infection
• dc.title A coordinated response at the transcriptome and interactome level is required to ensure uropathogenic Escherichia coli survival during bacteremia
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion