Genome analysis of Candida subhashii reveals its hybrid nature and dual mitochondrial genome conformations

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  • dc.contributor.author Mixão, Verónica
  • dc.contributor.author Hegedűsová, Eva
  • dc.contributor.author Saus Martínez, Ester
  • dc.contributor.author Pryszcz, Leszek Piotr, 1985-
  • dc.contributor.author Cillingová, Andrea
  • dc.contributor.author Nosek, Jozef
  • dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
  • dc.date.accessioned 2021-11-18T06:41:23Z
  • dc.date.available 2021-11-18T06:41:23Z
  • dc.date.issued 2021
  • dc.description.abstract Candida subhashii belongs to the CUG-Ser clade, a group of phylogenetically closely related yeast species that includes some human opportunistic pathogens, such as Candida albicans. Despite being present in the environment, C. subhashii was initially described as the causative agent of a case of peritonitis. Considering the relevance of whole-genome sequencing and analysis for our understanding of genome evolution and pathogenicity, we sequenced, assembled and annotated the genome of C. subhashii type strain. Our results show that C. subhashii presents a highly heterozygous genome and other signatures that point to a hybrid ancestry. The presence of functional pathways for assimilation of hydroxyaromatic compounds goes in line with the affiliation of this yeast with soil microbial communities involved in lignin decomposition. Furthermore, we observed that different clones of this strain may present circular or linear mitochondrial DNA. Re-sequencing and comparison of strains with differential mitochondrial genome topology revealed five candidate genes potentially associated with this conformational change: MSK1, SSZ1, ALG5, MRPL9 and OYE32.
  • dc.description.sponsorship This work was supported by the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie grant agreement No. H2020-MSCA-ITN-2014-642095. T.G. group also acknowledges support from the Spanish Ministry of Economy, Industry, and Competitiveness (MEIC) for the EMBL partnership, and grants ‘Centro de Excelencia Severo Ochoa 2013-2017’ SEV-2012-0208 and BFU2015-67107 co-founded by European Regional Development Fund (ERDF); from the CERCA Programme/Generalitat de Catalunya; from the Catalan Research Agency (AGAUR) SGR857, and grants from the European Union’s Horizon 2020 Research and Innovation Programme under the grant agreement ERC-2016-724173. T.G. also receives support from an INB Grant (PT17/0009/0023 - ISCIII-SGEFI/ERDF). J.N. group was supported by the Slovak Research and Development Agency (APVV-18-0239) and the Scientific Grant Agency of the Ministry of Education, Science, Research and Sport of the Slovak Republic (VEGA 1/0027/19).
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Mixão V, Hegedűsová E, Saus E, Pryszcz LP, Cillingová A, Nosek J, Gabaldón T. Genome analysis of Candida subhashii reveals its hybrid nature and dual mitochondrial genome conformations. DNA Res. 2021;28(3):dsab006. DOI: 10.1093/dnares/dsab006
  • dc.identifier.doi http://dx.doi.org/10.1093/dnares/dsab006
  • dc.identifier.issn 1340-2838
  • dc.identifier.uri http://hdl.handle.net/10230/49009
  • dc.language.iso eng
  • dc.publisher Oxford University Press
  • dc.relation.ispartof DNA Res. 2021;28(3):dsab006
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/642095
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/724173
  • dc.rights © The Author(s) 2021. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
  • dc.subject.keyword Candida subhashii
  • dc.subject.keyword Genome assembly
  • dc.subject.keyword Hybrid
  • dc.subject.keyword Metabolism of hydroxyaromatic compounds
  • dc.subject.keyword Mitochondria
  • dc.title Genome analysis of Candida subhashii reveals its hybrid nature and dual mitochondrial genome conformations
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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