Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation

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• dc.contributor.author Schaber, Jörgca
• dc.contributor.author Adrover Nadal, Miguel Angelca
• dc.contributor.author Eriksson, Emmaca
• dc.contributor.author Pelet, Sergeca
• dc.contributor.author Petelenz-Kurdziel, Elzbietaca
• dc.contributor.author Klein, Dagmaraca
• dc.contributor.author Posas Garriga, Francescca
• dc.contributor.author Goksör, Mattiasca
• dc.contributor.author Peter, Matthiasca
• dc.contributor.author Hohmann, Stefanca
• dc.contributor.author Klipp, E., 1965-ca
• dc.date.accessioned 2016-01-14T13:30:09Z
• dc.date.available 2016-01-14T13:30:09Z
• dc.date.issued 2010
• dc.description.abstract Parameterized models of biophysical and mechanical cell properties are important for predictive mathematical modeling of cellular processes. The concepts of turgor, cell wall elasticity, osmotically active volume, and intracellular osmolarity have been investigated for decades, but a consistent rigorous parameterization of these concepts is lacking. Here, we subjected several data sets of minimum volume measurements in yeast obtained after hyper-osmotic shock to a thermodynamic modeling framework. We estimated parameters for several relevant biophysical cell properties and tested alternative hypotheses about these concepts using a model discrimination approach. In accordance with previous reports, we estimated an average initial turgor of 0.6 ± 0.2 MPa and found that turgor becomes negligible at a relative volume of 93.3 ± 6.3% corresponding to an osmotic shock of 0.4 ± 0.2 Osm/l. At high stress levels (4 Osm/l), plasmolysis may occur. We found that the volumetric elastic modulus, a measure of cell wall elasticity, is 14.3 ± 10.4 MPa. Our model discrimination analysis suggests that other thermodynamic quantities affecting the intracellular water potential, for example the matrix potential, can be neglected under physiological conditions. The parameterized turgor models showed that activation of the osmosensing high osmolarity glycerol (HOG) signaling pathway correlates with turgor loss in a 1:1 relationship. This finding suggests that mechanical properties of the membrane trigger HOG pathway activation, which can be represented and quantitatively modeled by turgor.ca
• dc.description.sponsorship This work was supported via several projects funded by the European Commission: QUASI (Contract No. 503230 to SH, EK, FP and MP), CELLCOMPUT (Contract No. 043310 to SH, EK and FP), UNICELLSYS (Contract No. 201142 to SH, EK, FP, MP and MG), SYSTEMSBIOLOGY (Contract No. 514169 to SH and EK), and AMPKIN (Contract No. 518181 to SH and MG). In addition work was funded by grants from the Swedish Foundation for Strategic Research SSF (Bio-X to MG), the Swedish Research Council (project grants to SH and MG), the Carl Trygger Foundation (to MG), the Science Faculty, University of Gothenburg (to SH and MG), and the Swiss systemsX.ch (to MP)
• dc.format.mimetype application/pdfca
• dc.identifier.citation Schaber J, Adrover MA, Eriksson E, Pelet S, Petelenz-Kurdziel E, Klein D et al. Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation. Eur Biophys J. 2010;39(11):1547-56. DOI: 10.1007/s00249-010-0612-0ca
• dc.identifier.doi http://dx.doi.org/10.1007/s00249-010-0612-0
• dc.identifier.issn 0175-7571
• dc.identifier.uri http://hdl.handle.net/10230/25575
• dc.language.iso engca
• dc.publisher Springer Verlagca
• dc.relation.ispartof European biophysics journal. 2010;39(11):1547-56
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP6/503230
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP6/43310
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP6/201142
• dc.rights © The Author(s) 2010. This article is published with open access at Springerlink.comca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.keyword Turgor
• dc.subject.keyword Cell wall elasticity
• dc.subject.keyword Volumetric elastic modulus
• dc.subject.keyword High osmolarity glycerol (HOG) signaling
• dc.subject.keyword Plasmolysis
• dc.subject.keyword Model discrimination
• dc.subject.keyword Yeast
• dc.subject.other Membranes cel·lularsca
• dc.subject.other Saccharomyces cerevisiae -- Metabolismeca
• dc.title Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activationca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca