Bioconversion of stilbenes in genetically engineered root and cell cultures of tobacco
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- dc.contributor.author Hidalgo, Diegoca
- dc.contributor.author Martínez-Márquez, Ascensiónca
- dc.contributor.author Moyano Claramunt, Elisabetca
- dc.contributor.author Bru-Martínez, Roqueca
- dc.contributor.author Corchete, Purificaciónca
- dc.contributor.author Palazón, Javierca
- dc.date.accessioned 2017-06-20T12:05:14Z
- dc.date.available 2017-06-20T12:05:14Z
- dc.date.issued 2017
- dc.description.abstract It is currently possible to transfer a biosynthetic pathway from a plant to another organism. This system has been exploited to transfer the metabolic richness of certain plant species to other plants or even to more simple metabolic organisms such as yeast or bacteria for the production of high added value plant compounds. Another application is to bioconvert substrates into scarcer or biologically more interesting compounds, such as piceatannol and pterostilbene. These two resveratrol-derived stilbenes, which have very promising pharmacological activities, are found in plants only in small amounts. By transferring the human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) gene to tobacco hairy roots and cell cultures, we developed a system able to bioconvert exogenous t-resveratrol into piceatannol in quantities near to mg L−1. Similarly, after heterologous expression of resveratrol O-methyltransferase from Vitis vinifera (VvROMT) in tobacco hairy roots, the exogenous t-resveratrol was bioconverted into pterostilbene. We also observed that both bioconversions can take place in tobacco wild type hairy roots (pRiA4, without any transgene), showing that unspecific tobacco P450 hydroxylases and methyltransferases can perform the bioconversion of t-resveratrol to give the target compounds, albeit at a lower rate than transgenic roots.
- dc.description.sponsorship This work has been supported by a grant from the Spanish Ministry of Science and Innovation (BIO2014-51861-R), Generalitat de Catalunya (2014SGR215).
- dc.format.mimetype application/pdfca
- dc.identifier.citation Hidalgo D, Martínez-Márquez A, Moyano Claramunt E, Bru-Martínez R, Corchete P, Palazon J. Bioconversion of stilbenes in genetically engineered root and cell cultures of tobacco. Scientific Reports. 2017; 7: 45331. DOI: 10.1038/srep45331
- dc.identifier.doi http://dx.doi.org/10.1038/srep45331
- dc.identifier.issn 2045-2322
- dc.identifier.uri http://hdl.handle.net/10230/32380
- dc.language.iso eng
- dc.publisher Nature Publishing Groupca
- dc.relation.ispartof Scientific Reports. 2017;7:45331
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2014-51861-R
- dc.rights © Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Metabolic engineering
- dc.subject.keyword Molecular engineering in plants
- dc.title Bioconversion of stilbenes in genetically engineered root and cell cultures of tobaccoca
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion