Rapid reversible changes in compartments and local chromatin organization revealed by hyperosmotic shock
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- dc.contributor.author Amat, Ramon
- dc.contributor.author Böttcher, René
- dc.contributor.author Le Dily, François
- dc.contributor.author Vidal Ocabo, Enrique
- dc.contributor.author Quilez Oliete, Javier
- dc.contributor.author Cuartero, Yasmina
- dc.contributor.author Beato, Miguel
- dc.contributor.author Nadal Clanchet, Eulàlia de
- dc.contributor.author Posas Garriga, Francesc
- dc.date.accessioned 2019-03-06T08:36:52Z
- dc.date.available 2019-03-06T08:36:52Z
- dc.date.issued 2019
- dc.description.abstract Nuclear architecture is decisive for the assembly of transcriptional responses. However, how chromosome organization is dynamically modulated to permit rapid and transient transcriptional changes in response to environmental challenges remains unclear. Here we show that hyperosmotic stress disrupts different levels of chromosome organization, ranging from A/B compartment changes to reduction in the number and insulation of topologically associating domains (TADs). Concomitantly, transcription is greatly affected, TAD borders weaken, and RNA Polymerase II runs off from hundreds of transcription end sites. Stress alters the binding profiles of architectural proteins, which explains the disappearance of local chromatin organization. These processes are dynamic, and cells rapidly reconstitute their default chromatin conformation after stress removal, uncovering an intrinsic organization. Transcription is not required for local chromatin reorganization, while compartment recovery is partially transcription-dependent. Thus, nuclear organization in mammalian cells can be rapidly modulated by environmental changes in a reversible manner.
- dc.description.sponsorship The study was supported by grants from the Spanish Ministry of Economy and Competitiveness (BFU2015-64437-P and FEDER, BFU2014-52125-REDT, and BFU2014-51672-REDC to F.P.; BFU2017-85152-P and FEDER to E.d.N.), the Catalan Government (2017 SGR 799), the Fundación Botín, and the Banco Santander through its Santander Universities Global Division to F.P. and the Unidad de Excelencia Maria de Maeztu, MDM-2014-0370. F.P. is recipient of an ICREA Acadèmia (Generalitat de Catalunya). M.B. received the support of the European Research Council (ERC Synergy Grant 4D Genome 609989) and CERCA.
- dc.format.mimetype application/pdf
- dc.identifier.citation Amat R, Böttcher R, Le Dily F, Vidal E, Quilez J, Cuartero Y, Beato M, de Nadal E, Posas F. Rapid reversible changes in compartments and local chromatin organization revealed by hyperosmotic shock. Genome Res. 2019;29(1):18-28. DOI: 10.1101/gr.238527.118
- dc.identifier.doi http://dx.doi.org/10.1101/gr.238527.118
- dc.identifier.issn 1088-9051
- dc.identifier.uri http://hdl.handle.net/10230/36750
- dc.language.iso eng
- dc.publisher Cold Spring Harbor Laboratory Press (CSHL Press)
- dc.relation.ispartof Genome Research. 2019;29(1):18-28
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-64437-P
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-52125-REDT
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-51672-REDC
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/609989
- dc.rights © 2019 Amat et al.; Published by Cold Spring Harbor Laboratory Press. This article, published in Genome Research, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
- dc.title Rapid reversible changes in compartments and local chromatin organization revealed by hyperosmotic shock
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion