HeT-A_pi1, a piRNA target sequence in the drosophila telomeric retrotransposon HeT-A, is extremely conserved across copies and species
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- dc.contributor.author Petit, Nataliaca
- dc.contributor.author Piñeyro, Davidca
- dc.contributor.author López-Panades, Elisendaca
- dc.contributor.author Casacuberta Suñer, Elena, 1972-ca
- dc.contributor.author Navarro i Cuartiellas, Arcadi, 1969-ca
- dc.date.accessioned 2015-05-08T07:19:32Z
- dc.date.available 2015-05-08T07:19:32Z
- dc.date.issued 2012ca
- dc.description.abstract The maintenance of the telomeres in Drosophila species depends on the transposition of the non-LTR retrotransposons HeT-A, TAHRE and TART. HeT-A and TART elements have been found in all studied species of Drosophila suggesting that their function has been maintained for more than 60 million years. Of the three elements, HeT-A is by far the main component of D. melanogaster telomeres and, unexpectedly for an element with an essential role in telomere elongation, the conservation of the nucleotide sequence of HeT-A is very low. In order to better understand the function of this telomeric retrotransposon, we studied the degree of conservation along HeT-A copies. We identified a small sequence within the 3′ UTR of the element that is extremely conserved among copies of the element both, within D. melanogaster and related species from the melanogaster group. The sequence corresponds to a piRNA target in D. melanogaster that we named HeT-A_pi1. Comparison with piRNA target sequences from other Drosophila retrotransposons showed that HeT-A_pi1 is the piRNA target in the Drosophila genome with the highest degree of conservation among species from the melanogaster group. The high conservation of this piRNA target in contrast with the surrounding sequence, suggests an important function of the HeT-A_pi1 sequence in the co-evolution of the HeT-A retrotransposon and the Drosophila genomeen
- dc.description.sponsorship This work was supported by grants from the Spanish Ministry of Science and Innovation BFU2009-08318/BMC to EC and BFU2009-13409-C02-02 to AN. Funding was also provided by the Spanish National Institute for Bioinformatics (www.inb.org). NP is supported by JAE-doc09_01394 from Consejo Superior de Investigaciones Científicas (CSIC) and FEDER. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscripten
- dc.format.mimetype application/pdfca
- dc.identifier.citation Petit N, Pineyro D, Lopez-Panades E, Casacuberta E, Navarro A. HeT-A_pi1, a piRNA target sequence in the drosophila telomeric retrotransposon HeT-A, is extremely conserved across copies and species. PLoS ONE. 2012;7(5):e37405. DOI: 10.1371/journal.pone.0037405ca
- dc.identifier.doi http://dx.doi.org/10.1371/journal.pone.0037405
- dc.identifier.issn 1932-6203ca
- dc.identifier.uri http://hdl.handle.net/10230/23541
- dc.language.iso engca
- dc.publisher Public Library of Science (PLoS)ca
- dc.relation.ispartof PLoS ONE. 2012;7(5):e37405
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-08318
- dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2009-13409
- dc.rights © 2012 Petit et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- dc.rights.accessRights info:eu-repo/semantics/openAccessca
- dc.subject.other Drosòfila -- Genèticaca
- dc.title HeT-A_pi1, a piRNA target sequence in the drosophila telomeric retrotransposon HeT-A, is extremely conserved across copies and speciesen
- dc.type info:eu-repo/semantics/articleca
- dc.type.version info:eu-repo/semantics/publishedVersionca