Delegating sex: differential gene expression in stolonizing syllids uncovers the hormonal control of reproduction

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• dc.contributor.author Álvarez Campos, Patricia
• dc.contributor.author Kenny, Nathan J.
• dc.contributor.author Verdes, Aida
• dc.contributor.author Fernández, Rosa
• dc.contributor.author Novo, Marta
• dc.contributor.author Giribet, Gonzalo
• dc.contributor.author Riesgo, Ana
• dc.date.accessioned 2020-03-26T13:57:40Z
• dc.date.available 2020-03-26T13:57:40Z
• dc.date.issued 2019
• dc.description.abstract Stolonization in syllid annelids is a unique mode of reproduction among animals. During the breeding season, a structure resembling the adult but containing only gametes, called stolon, is formed generally at the posterior end of the animal. When stolons mature, they detach from the adult and gametes are released into the water column. The process is synchronized within each species, and it has been reported to be under environmental and endogenous control, probably via endocrine regulation. To further understand reproduction in syllids and to elucidate the molecular toolkit underlying stolonization, we generated Illumina RNA-seq data from different tissues of reproductive and nonreproductive individuals of Syllis magdalena and characterized gene expression during the stolonization process. Several genes involved in gametogenesis (ovochymase, vitellogenin, testis-specific serine/threonine-kinase), immune response (complement receptor 2), neuronal development (tyrosine-protein kinase Src42A), cell proliferation (alpha-1D adrenergic receptor), and steroid metabolism (hydroxysteroid dehydrogenase 2) were found differentially expressed in the different tissues and conditions analyzed. In addition, our findings suggest that several neurohormones, such as methyl farnesoate, dopamine, and serotonin, might trigger stolon formation, the correct maturation of gametes and the detachment of stolons when gametogenesis ends. The process seems to be under circadian control, as indicated by the expression patterns of r-opsins. Overall, our results shed light into the genes that orchestrate the onset of gamete formation and improve our understanding of how some hormones, previously reported to be involved in reproduction and metamorphosis processes in other invertebrates, seem to also regulate reproduction via stolonization.
• dc.description.sponsorship This research received funding from the European Union’s (European Atomic Energy Community’s) Seventh Framework Program (FP7/2007–2013; FP7/2007–2011) under grant agreement 227799 to P.A.-C. Sequencing and analyses were conducted with internal MCZ funds to G.G. and with the support of the Center for Systems Biology and the Research Computing group, both from the Faculty of Arts and Sciences (Harvard University), and with internal funds from the Department of Life Sciences (Natural History Museum of London) to AR. One anonymous reviewer and Associate Editor Mandë Holford provided constructive criticism which helped to improve this study.
• dc.format.mimetype application/pdf
• dc.identifier.citation Álvarez-Campos P, Kenny NJ, Verdes A, Fernández R, Novo M, Giribet G, Riesgo A. Delegating sex: differential gene expression in stolonizing syllids uncovers the hormonal control of reproduction. Genome Biol Evol. 2019; 11(1):295-318. DOI: 10.1093/gbe/evy265
• dc.identifier.doi http://dx.doi.org/10.1093/gbe/evy265
• dc.identifier.issn 1759-6653
• dc.identifier.uri http://hdl.handle.net/10230/44053
• dc.language.iso eng
• dc.publisher Oxford University Press
• dc.relation.ispartof Genome Biol Evol. 2019; 11(1):295-318
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/227799
• dc.rights © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Transcriptomics
• dc.subject.keyword Stolonizing syllids
• dc.subject.keyword Reproduction
• dc.subject.keyword Hormonal control
• dc.title Delegating sex: differential gene expression in stolonizing syllids uncovers the hormonal control of reproduction
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion