Transcription start site associated RNAs in bacteria

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  • dc.contributor.author Yus, Evaca
  • dc.contributor.author Güell Cargol, Marc, 1982-ca
  • dc.contributor.author Vivancos Prellezo, Anaca
  • dc.contributor.author Chen, Wei-Huaca
  • dc.contributor.author Lluch-Senar, Maria 1982-ca
  • dc.contributor.author Delgado Blanco, Javierca
  • dc.contributor.author Gavin, Anne-Claudeca
  • dc.contributor.author Bork, Peerca
  • dc.contributor.author Serrano Pubull, Luis, 1982-ca
  • dc.date.accessioned 2014-04-29T08:44:37Z
  • dc.date.available 2014-04-29T08:44:37Z
  • dc.date.issued 2012ca
  • dc.description.abstract Here, we report the genome-wide identification of small RNAs associated with transcription start sites (TSSs), termed tssRNAs, in Mycoplasma pneumoniae. tssRNAs were also found to be present in a different bacterial phyla, Escherichia coli. Similar to the recently identified promoter-associated tiny RNAs (tiRNAs) in eukaryotes, tssRNAs are associated with active promoters. Evidence suggests that these tssRNAs are distinct from previously described abortive transcription RNAs. ssRNAs have an average size of 45 bases and map exactly to the beginning of cognate full-length transcripts and to cryptic TSSs. Expression of bacterial tssRNAs requires factors other than the standard RNA polymerase holoenzyme. We have found that the RNA polymerase is halted at tssRNA positions in vivo, which may indicate that a pausing mechanism exists to prevent transcription in the absence of genes. These results suggest that small RNAs associated with TSSs could be a universal feature of bacterial transcription.
  • dc.description.sponsorship This work was supported by an European Research council (ERC) advanced grant, the Fundación Marcelino Botín, the Spanish Ministry of Research and ICREA (Institució Catalana de Recerca i Estudis Avançats)
  • dc.format.mimetype application/pdfca
  • dc.identifier.citation Yus E, Güell M, Vivancos AP, Chen WH, Lluch-Senar M, Delgado J et al. Transcription start site associated RNAs in bacteria. Mol Syst Biol. 2012; 8:585. DOI: 10.1038/msb.2012.16ca
  • dc.identifier.doi http://dx.doi.org/10.1038/msb.2012.16
  • dc.identifier.issn 1744-4292ca
  • dc.identifier.uri http://hdl.handle.net/10230/22229
  • dc.language.iso engca
  • dc.publisher Nature Publishing Groupca
  • dc.relation.ispartof Molecular Systems Biology. 2012;8:585
  • dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/232913
  • dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike Licenseca
  • dc.rights.accessRights info:eu-repo/semantics/openAccessca
  • dc.rights.uri http://creativecommons.org/licenses/by/3.0/
  • dc.subject.other RNA
  • dc.subject.other Microbiologia
  • dc.title Transcription start site associated RNAs in bacteriaca
  • dc.type info:eu-repo/semantics/articleca
  • dc.type.version info:eu-repo/semantics/publishedVersionca

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