Control of Ubp3 ubiquitin protease activity by the Hog1 SAPK modulates transcription upon osmostress

Solé, Carme; Nadal Ribelles, Mariona, 1984-; Kraft, Claudine; Peter, Matthias; Posas Garriga, Francesc; Nadal Clanchet, Eulàlia de

Control of Ubp3 ubiquitin protease activity by the Hog1 SAPK modulates transcription upon osmostress

Mostra el registre complet Registre parcial de l'ítem

dc.contributor.author Solé, Carmeca
dc.contributor.author Nadal Ribelles, Mariona, 1984-ca
dc.contributor.author Kraft, Claudineca
dc.contributor.author Peter, Matthiasca
dc.contributor.author Posas Garriga, Francescca
dc.contributor.author Nadal Clanchet, Eulàlia deca
dc.date.accessioned 2015-12-17T15:44:29Z
dc.date.available 2015-12-17T15:44:29Z
dc.date.issued 2011
dc.description.abstract Protein ubiquitylation is a key process in the regulation of many cellular processes. The balance between the activity of ubiquitin ligases and that of proteases controls the level of ubiquitylation. In response to extracellular stimuli, stress-activated protein kinases (SAPK) modulate gene expression to maximize cell survival. In yeast, the Hog1 SAPK has a key role in reprogramming the gene expression pattern required for cell survival upon osmostress. Here, we show that the Ubp3 ubiquitin protease is a target for the Hog1 SAPK to modulate gene expression. ubp3 mutant cells are defective in expression of osmoresponsive genes. Hog1 interacts with and phosphorylates Ubp3 at serine 695, which is essential to determine the extent of transcriptional activation in response to osmostress. Furthermore, Ubp3 is recruited to osmoresponsive genes to modulate transcriptional initiation as well as elongation. Therefore, Ubp3 activity responds to external stimuli and is required for transcriptional activation upon osmostress.ca
dc.description.sponsorship MN is the recipient of an FIS (Spanish Government) fellowship and FP is the recipient of an ICREA Acadèmia (Generalitat de Catalunya). This work was supported by Fundación Marcelino Botín (FMB) and grants from the Spanish Ministry of Science and Innovation (BFU2008-00530 to EN and BIO2009-07762 to FP) and the FP7 (UNICELLSYS) framework programme.
dc.format.mimetype application/pdfca
dc.identifier.citation Solé C, Nadal-Ribelles M, Kraft C, Peter M, Posas F, de Nadal E. Control of Ubp3 ubiquitin protease activity by the Hog1 SAPK modulates transcription upon osmostress. EMBO journal. 2011;30(16):3274-84. DOI: 10.1038/emboj.2011.227ca
dc.identifier.doi http://dx.doi.org/10.1038/emboj.2011.227
dc.identifier.issn 0261-4189
dc.identifier.uri http://hdl.handle.net/10230/25459
dc.language.iso engca
dc.publisher Nature Publishing Groupca
dc.relation.ispartof EMBO journal. 2011;30(16):3274-84
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/201142
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2008-00530
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2009-07762
dc.rights.accessRights info:eu-repo/semantics/openAccessca
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ca
dc.subject.keyword Gene expression
dc.subject.keyword Hog1
dc.subject.keyword Osmostress
dc.subject.keyword SAPK
dc.subject.keyword Ubp3
dc.subject.other RNA missatgerca
dc.subject.other Saccharomyces cerevisiae -- Metabolismeca
dc.subject.other Endopeptidases -- Fisiologia patològicaca
dc.title Control of Ubp3 ubiquitin protease activity by the Hog1 SAPK modulates transcription upon osmostressca
dc.type info:eu-repo/semantics/articleca
dc.type.version info:eu-repo/semantics/publishedVersionca

Col·leccions

Articles (Departament de Medicina i Ciències de la Vida)
Documents OpenAIRE (Open Access Infrastructure for Research in Europe)