PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells
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- dc.contributor.author Jain, Payal, 1986-
- dc.contributor.author Ballaré, Cecilia Julia
- dc.contributor.author Blanco, Enrique
- dc.contributor.author Vizán, Pedro
- dc.contributor.author Di Croce, Luciano
- dc.date.accessioned 2020-05-07T11:02:51Z
- dc.date.available 2020-05-07T11:02:51Z
- dc.date.issued 2020
- dc.description.abstract The Polycomb-like protein PHF19/PCL3 associates with PRC2 and mediates its recruitment to chromatin in embryonic stem cells. PHF19 is also overexpressed in many cancers. However, neither PHF19 targets nor misregulated pathways involving PHF19 are known. Here, we investigate the role of PHF19 in prostate cancer cells. We find that PHF19 interacts with PRC2 and binds to PRC2 targets on chromatin. PHF19 target genes are involved in proliferation, differentiation, angiogenesis, and extracellular matrix organization. Depletion of PHF19 triggers an increase in MTF2/PCL2 chromatin recruitment, with a genome-wide gain in PRC2 occupancy and H3K27me3 deposition. Transcriptome analysis shows that PHF19 loss promotes deregulation of key genes involved in growth, metastasis, invasion, and of factors that stimulate blood vessels formation. Consistent with this, PHF19 silencing reduces cell proliferation, while promotes invasive growth and angiogenesis. Our findings reveal a role for PHF19 in controlling the balance between cell proliferation and invasiveness in prostate cancer.
- dc.description.sponsorship This work was suported by the Spanish Ministry of Science and Innovation (BFU2016-75008-P), the Fundación Vencer El Cáncer, the European Regional Development Fund, the Fundación Científica Asociación Española Contra el Cáncer, the Fundació la Marató de TV3, the Agència de Gestió d’Ajuts Universitaris i de Recerca, the Generalitat de Catalunya (Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement), the Instituto de Salud Carlos III, the European Molecular Biology Laboratory, the Centro de Excelencia Severo Ochoa and the Generalitat de Catalunya (Centres de Recerca de Catalunya)
- dc.format.mimetype application/pdf
- dc.identifier.citation Jain P, Ballare C, Blanco E, Vizan P, Di Croce L. PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells. Elife. 2020 Mar 10; 9. pii: e51373. DOI: 10.7554/eLife.51373
- dc.identifier.doi http://dx.doi.org/10.7554/eLife.51373
- dc.identifier.issn 2050-084X
- dc.identifier.uri http://hdl.handle.net/10230/44449
- dc.language.iso eng
- dc.publisher eLife
- dc.relation.ispartof Elife. 2020 Mar 10;9. pii: e51373
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2016-75008-P
- dc.rights © Payal Jain et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.other Pròstata -- Càncer
- dc.subject.other Genètica
- dc.title PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion