Diverse families of transposable elements affect the transcriptional regulation of stress-response genes in Drosophila melanogaster
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- dc.contributor.author Villanueva Cañas, José Luis, 1984-
- dc.contributor.author Horvath, Vivien
- dc.contributor.author Aguilera, Laura
- dc.contributor.author González, Josefa
- dc.date.accessioned 2020-07-14T06:13:48Z
- dc.date.available 2020-07-14T06:13:48Z
- dc.date.issued 2019
- dc.description.abstract Although transposable elements are an important source of regulatory variation, their genome-wide contribution to the transcriptional regulation of stress-response genes has not been studied yet. Stress is a major aspect of natural selection in the wild, leading to changes in the transcriptional regulation of a variety of genes that are often triggered by one or a few transcription factors. In this work, we take advantage of the wealth of information available for Drosophila melanogaster and humans to analyze the role of transposable elements in six stress regulatory networks: immune, hypoxia, oxidative, xenobiotic, heat shock, and heavy metal. We found that transposable elements were enriched for caudal, dorsal, HSF, and tango binding sites in D. melanogaster and for NFE2L2 binding sites in humans. Taking into account the D. melanogaster population frequencies of transposable elements with predicted binding motifs and/or binding sites, we showed that those containing three or more binding motifs/sites are more likely to be functional. For a representative subset of these TEs, we performed in vivo transgenic reporter assays in different stress conditions. Overall, our results showed that TEs are relevant contributors to the transcriptional regulation of stress-response genes.
- dc.description.sponsorship Ministerio de Economia y Competitividad [BFU2014-57779-P]; Ministerio de Ciencia, Innovación y Universidades/AEI [BFU2017-82937-P]; European Commission [H2020-ERC-2014-CoG-647900]. Funding for open access charge: European Commission [H2020-ERC-2014-CoG-647900].
- dc.format.mimetype application/pdf
- dc.identifier.citation Villanueva-Cañas JL, Horvath V, Aguilera L, González J. Diverse families of transposable elements affect the transcriptional regulation of stress-response genes in Drosophila melanogaster. Nucleic Acids Res. 2019; 47(13):6842-57. DOI: 10.1093/nar/gkz490
- dc.identifier.doi http://dx.doi.org/10.1093/nar/gkz490
- dc.identifier.issn 0305-1048
- dc.identifier.uri http://hdl.handle.net/10230/45112
- dc.language.iso eng
- dc.publisher Oxford University Press
- dc.relation.ispartof Nucleic Acids Res. 2019; 47(13):6842-57
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/647900
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-57779-P
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BFU2017-82937-P
- dc.rights © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
- dc.title Diverse families of transposable elements affect the transcriptional regulation of stress-response genes in Drosophila melanogaster
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion