Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis
Mostra el registre complet Registre parcial de l'ítem
- dc.contributor.author Colomé, Núria
- dc.contributor.author Chiva, Cristina
- dc.contributor.author Sabidó Aguadé, Eduard, 1981-
- dc.contributor.author EuPA
- dc.date.accessioned 2022-04-04T06:31:21Z
- dc.date.available 2022-04-04T06:31:21Z
- dc.date.issued 2022
- dc.description.abstract Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool in biological and biomedical research. However, there are several factors that make the global study of the phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry of the phosphorylated species and the need to retrieve residue specific information require particular attention on sample preparation, data acquisition and processing to ensure reproducibility, qualitative and quantitative robustness and ample phosphoproteome coverage in phosphoproteomic workflows. Aiming to investigate the effect of different variables in the performance of proteome wide phosphoprotein analysis protocols, ProteoRed-ISCIII and EuPA launched the Proteomics Multicentric Experiment 11 (PME11). A reference sample consisting of a yeast protein extract spiked in with different amounts of a phosphomix standard (Sigma/Merck) was distributed to 31 laboratories around the globe. Thirty-six datasets from 23 laboratories were analyzed. Our results indicate the suitability of the PME11 reference sample to benchmark and optimize phosphoproteomics strategies, weighing the influence of different factors, as well as to rank intra and inter laboratory performance.
- dc.description.sponsorship Funding: ProteoRed, PRB3 is supported by grant PT17/0019/0001, of the PE I+D+i 2013-2016, funded by ISCIII and ERDF
- dc.format.mimetype application/pdf
- dc.identifier.citation Colomé N, Abian J, Aloria K, Arizmendi JM, Barceló-Batllori S, Braga-Lagache S et al. Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis. J Proteomics. 2022 Jan 16;251:104409. DOI: 10.1016/j.jprot.2021.104409
- dc.identifier.doi http://dx.doi.org/10.1016/j.jprot.2021.104409
- dc.identifier.issn 1874-3919
- dc.identifier.uri http://hdl.handle.net/10230/52822
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof J Proteomics. 2022 Jan 16;251:104409
- dc.rights © 2021 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.other Fosforilació
- dc.subject.other Fosfoproteïnes
- dc.subject.other Proteòmica
- dc.title Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion