Snail1 transcription factor controls telomere transcription and integrity

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• dc.contributor.author Mazzolini, Roccoca
• dc.contributor.author Gonzàlez Busqué, Núria, 1980-ca
• dc.contributor.author Garcia-Garijo, Andreaca
• dc.contributor.author Millanes Romero, Alba, 1986-ca
• dc.contributor.author Peiró Sales, Sandraca
• dc.contributor.author Smith, Susanca
• dc.contributor.author García de Herreros, Antonioca
• dc.contributor.author Canudas i Puig, Sílviaca
• dc.date.accessioned 2018-07-05T07:23:05Z
• dc.date.available 2018-07-05T07:23:05Z
• dc.date.issued 2018
• dc.description.abstract Besides controlling epithelial-to-mesenchymal transition (EMT) and cell invasion, the Snail1 transcriptional factor also provides cells with cancer stem cell features. Since telomere maintenance is essential for stemness, we have examined the control of telomere integrity by Snail1. Fluorescence in situ hybridization (FISH) analysis indicates that Snail1-depleted mouse mesenchymal stem cells (MSC) have both a dramatic increase of telomere alterations and shorter telomeres. Remarkably, Snail1-deficient MSC present higher levels of both telomerase activity and the long non-coding RNA called telomeric repeat-containing RNA (TERRA), an RNA that controls telomere integrity. Accordingly, Snail1 expression downregulates expression of the telomerase gene (TERT) as well as of TERRA 2q, 11q and 18q. TERRA and TERT are transiently downregulated during TGFβ-induced EMT in NMuMG cells, correlating with Snail1 expression. Global transcriptome analysis indicates that ectopic expression of TERRA affects the transcription of some genes induced during EMT, such as fibronectin, whereas that of TERT does not modify those genes. We propose that Snail1 repression of TERRA is required not only for telomere maintenance but also for the expression of a subset of mesenchymal genes.
• dc.format.mimetype application/pdf
• dc.identifier.citation Mazzolini R, Gonzàlez N, Garcia-Garijo A, Millanes-Romero A, Peiró S, Smith S. et al. Snail1 transcription factor controls telomere transcription and integrity. Nucleic Acids Res. 2018 Jan 9;46(1):146-58. DOI: 10.1093/nar/gkx958
• dc.identifier.doi http://dx.doi.org/10.1093/nar/gkx958
• dc.identifier.issn 0305-1048
• dc.identifier.uri http://hdl.handle.net/10230/35036
• dc.language.iso eng
• dc.publisher Oxford University Pressca
• dc.relation.ispartof Nucleic Acids Research. 2018 Jan 9;46(1):146-58
• dc.rights This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact moc.puo@snoissimrep.slanruoj
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
• dc.subject.other Telòmer
• dc.subject.other Cèl·lules epitelials
• dc.subject.other Càncer
• dc.title Snail1 transcription factor controls telomere transcription and integrityca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/acceptedVersion