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The zinc-finger protein Z4 cooperates with condensin II to regulate somatic chromosome pairing and 3D chromatin organization

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dc.contributor.author Puerto, Marta
dc.contributor.author Shukla, Mamta
dc.contributor.author Bujosa, Paula
dc.contributor.author Pérez Roldán, Juan
dc.contributor.author Torràs Llort, Mònica
dc.contributor.author Tamirisa, Srividya
dc.contributor.author Carbonell, Albert
dc.contributor.author Solé, Carme
dc.contributor.author Puspo, Joynob Akter
dc.contributor.author Cummings, Christopher
dc.contributor.author Nadal Clanchet, Eulàlia de
dc.contributor.author Posas Garriga, Francesc
dc.contributor.author Azorín Marín, Fernando
dc.contributor.author Rowley, M. Jordan
dc.date.accessioned 2024-07-17T06:11:10Z
dc.date.available 2024-07-17T06:11:10Z
dc.date.issued 2024
dc.identifier.citation Puerto M, Shukla M, Bujosa P, Pérez-Roldán J, Torràs-Llort M, Tamirisa S, et al. The zinc-finger protein Z4 cooperates with condensin II to regulate somatic chromosome pairing and 3D chromatin organization. Nucleic Acids Res. 2024 Jun 10;52(10):5596-609. DOI: 10.1093/nar/gkae198
dc.identifier.issn 0305-1048
dc.identifier.uri http://hdl.handle.net/10230/60768
dc.description.abstract Chromosome pairing constitutes an important level of genome organization, yet the mechanisms that regulate pairing in somatic cells and the impact on 3D chromatin organization are still poorly understood. Here, we address these questions in Drosophila, an organism with robust somatic pairing. In Drosophila, pairing preferentially occurs at loci consisting of numerous architectural protein binding sites (APBSs), suggesting a role of architectural proteins (APs) in pairing regulation. Amongst these, the anti-pairing function of the condensin II subunit CAP-H2 is well established. However, the factors that regulate CAP-H2 localization and action at APBSs remain largely unknown. Here, we identify two factors that control CAP-H2 occupancy at APBSs and, therefore, regulate pairing. We show that Z4, interacts with CAP-H2 and is required for its localization at APBSs. We also show that hyperosmotic cellular stress induces fast and reversible unpairing in a Z4/CAP-H2 dependent manner. Moreover, by combining the opposite effects of Z4 depletion and osmostress, we show that pairing correlates with the strength of intrachromosomal 3D interactions, such as active (A) compartment interactions, intragenic gene-loops, and polycomb (Pc)-mediated chromatin loops. Altogether, our results reveal new players in CAP-H2-mediated pairing regulation and the intimate interplay between inter-chromosomal and intra-chromosomal 3D interactions.
dc.description.sponsorship MICIN/AEI 10.13039/501100011033 [PGC2018-094538-B-100 and PID2021-123303NB-I00], ‘FEDER, una manera de hacer Europa’ and the Generalitat de Catalunya [SGR2014-204 and SGR2017-475] to F.A.; Spanish Ministry of Economy and Competitiveness [PID2021-124723NB-C21 and PID2021-124723NB-C22], Unidad de Excelencia Maria de Maeztu [MDM-2014-0370] to UPF and ICREA Acadèmia (Catalan Government) to F.P. and E.N. European Union's Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie [754510] to S.T.; National Institutes of Health Pathway to Independence Award [R00GM1287671] and NIH Maximizing Investigators’ Research Award [R35GM147467] to M.J.R.; M.P., P.B., J.P.-R. acknowledge receipt of FPI fellowships from MICIN/AEI [10.13039/501100011033] and ‘FEDER, una manera de hacer Europa’. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. Funding for open access charge: NIH [MIRA R35GM147467].
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartof Nucleic Acids Res. 2024 Jun 10;52(10):5596-609
dc.rights © The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/
dc.subject.other Zinc
dc.subject.other Cromatina
dc.subject.other Cromosomes
dc.title The zinc-finger protein Z4 cooperates with condensin II to regulate somatic chromosome pairing and 3D chromatin organization
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1093/nar/gkae198
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/754510
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-094538-B-100
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-123303NB-I00
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-124723NB-C21
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-124723NB-C22
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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