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SARS-CoV-2 seroprevalence study in pediatric patients and health care workers using multiplex antibody immunoassays

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dc.contributor.author Prados de la Torre, Esther
dc.contributor.author Obando, Ignacio
dc.contributor.author Vidal, Marta
dc.contributor.author Felipe, Beatriz de
dc.contributor.author Aguilar, Ruth
dc.contributor.author Izquierdo, Luis
dc.contributor.author Carolis, Carlo
dc.contributor.author Olbrich, Peter
dc.contributor.author Capilla-Miranda, Ana
dc.contributor.author Serra, Pau
dc.contributor.author Santamaria, Pere
dc.contributor.author Blanco-Lobo, Pilar
dc.contributor.author Moncunill, Gemma
dc.contributor.author Rodríguez-Ortega, Manuel J.
dc.contributor.author Dobaño, Carlota
dc.date.accessioned 2022-11-10T06:49:53Z
dc.date.available 2022-11-10T06:49:53Z
dc.date.issued 2022
dc.identifier.citation Prados de la Torre E, Obando I, Vidal M, de Felipe B, Aguilar R, Izquierdo L, Carolis C, Olbrich P, Capilla-Miranda A, Serra P, Santamaria P, Blanco-Lobo P, Moncunill G, Rodríguez-Ortega MJ, Dobaño C. SARS-CoV-2 seroprevalence study in pediatric patients and health care workers using multiplex antibody immunoassays. Viruses. 2022 Sep 14;14(9):2039. DOI: 10.3390/v14092039
dc.identifier.issn 1999-4915
dc.identifier.uri http://hdl.handle.net/10230/54778
dc.description.abstract SARS-CoV-2 infection has become a global health problem specially exacerbated with the continuous appearance of new variants. Healthcare workers (HCW) have been one of the most affected sectors. Children have also been affected, and although infection generally presents as a mild disease, some have developed the Pediatric Inflammatory Multisystem Syndrome Temporally Associated with SARS-CoV-2 (PIMS-TS). We recruited 190 adults (HCW and cohabitants, April to June 2020) and 57 children (April 2020 to September 2021), of whom 12 developed PIMS-TS, in a hospital-based study in Spain. Using an in-house Luminex assay previously validated, antibody levels were measured against different spike and nucleocapsid SARS-CoV-2 proteins, including the receptor-binding domain (RBD) of the Alpha, Beta, Gamma, and Delta variants of concern (VoC). Seropositivity rates obtained from children and adults, respectively, were: 49.1% and 11% for IgG, 45.6% and 5.8% for IgA, and 35.1% and 7.3% for IgM. Higher antibody levels were detected in children who developed PIMS-TS compared to those who did not. Using the COVID-19 IgM/IgA ELISA (Vircell, S.L.) kit, widely implemented in Spanish hospitals, a high number of false positives and lower seroprevalences compared with the Luminex estimates were found, indicating a significantly lower specificity and sensitivity. Comparison of antibody levels against RBD-Wuhan versus RBD-VoCs indicated that the strongest positive correlations for all three isotypes were with RBD-Alpha, while the lowest correlations were with RBD-Delta for IgG, RBD-Gamma for IgM, and RBD-Beta for IgA. This study highlights the differences in antibody levels between groups with different demographic and clinical characteristics, as well as reporting the IgG, IgM, and IgA response to RBD VoC circulating at the study period.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartof Viruses. 2022 Sep 14;14(9):2039
dc.rights © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.title SARS-CoV-2 seroprevalence study in pediatric patients and health care workers using multiplex antibody immunoassays
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.3390/v14092039
dc.subject.keyword COVID-19
dc.subject.keyword PIMS-TS
dc.subject.keyword SARS-CoV-2
dc.subject.keyword Antibody
dc.subject.keyword Antigen
dc.subject.keyword Children
dc.subject.keyword Cohort
dc.subject.keyword Healthcare workers
dc.subject.keyword Seropositivity
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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