Welcome to the UPF Digital Repository

Alteration in the Culex pipiens transcriptome reveals diverse mechanisms of the mosquito immune system implicated upon Rift Valley fever phlebovirus exposure

Show simple item record

dc.contributor.author Núñez, Ana I.
dc.contributor.author Esteve-Codina, Anna
dc.contributor.author Gómez-Garrido, Jèssica
dc.contributor.author Brustolin, Marco
dc.contributor.author Talavera, Sandra
dc.contributor.author Berdugo, Miguel
dc.contributor.author Dabad, Marc
dc.contributor.author Alioto, Tyler
dc.contributor.author Bensaid, Albert
dc.contributor.author Busquets, Núria
dc.date.accessioned 2022-06-13T08:55:43Z
dc.date.available 2022-06-13T08:55:43Z
dc.date.issued 2020
dc.identifier.citation Núñez AI, Esteve-Codina A, Gómez-Garrido J, Brustolin M, Talavera S, Berdugo M et al. Alteration in the Culex pipiens transcriptome reveals diverse mechanisms of the mosquito immune system implicated upon Rift Valley fever phlebovirus exposure. PLoS Negl Trop Dis. 2020 Dec 10;14(12):e0008870. DOI:10.1371/journal.pntd.0008870
dc.identifier.issn 1935-2727
dc.identifier.uri http://hdl.handle.net/10230/53469
dc.description.abstract Rift Valley fever phlebovirus (RVFV) causes an emerging zoonotic disease and is mainly transmitted by Culex and Aedes mosquitoes. While Aedes aegypti-dengue virus (DENV) is the most studied model, less is known about the genes involved in infection-responses in other mosquito-arboviruses pairing. The main objective was to investigate the molecular responses of Cx. pipiens to RVFV exposure focusing mainly on genes implicated in innate immune responses. Mosquitoes were fed with blood spiked with RVFV. The fully-engorged females were pooled at 3 different time points: 2 hours post-exposure (hpe), 3- and 14-days post-exposure (dpe). Pools of mosquitoes fed with non-infected blood were also collected for comparisons. Total RNA from each mosquito pool was subjected to RNA-seq analysis and a de novo transcriptome was constructed. A total of 451 differentially expressed genes (DEG) were identified. Most of the transcriptomic alterations were found at an early infection stage after RVFV exposure. Forty-eight DEG related to immune infection-response were characterized. Most of them were related with the RNAi system, Toll and IMD pathways, ubiquitination pathway and apoptosis. Our findings provide for the first time a comprehensive view on Cx. pipiens-RVFV interactions at the molecular level. The early depletion of RNAi pathway genes at the onset of the RVFV infection would allow viral replication in mosquitoes. While genes from the Toll and IMD immune pathways were altered in response to RVFV none of the DEG were related to the JAK/STAT pathway. The fact that most of the DEG involved in the Ubiquitin-proteasome pathway (UPP) or apoptosis were found at an early stage of infection would suggest that apoptosis plays a regulatory role in infected Cx. pipiens midguts. This study provides a number of target genes that could be used to identify new molecular targets for vector control.
dc.description.sponsorship This study was funded by CERCA Programme/Generalitat de Catalunya (http://cerca.cat/) and NB received an award to perform this work from Ministerio de Economía, Industria y Competitividad, Gobierno de España (grant no. MINECO AGL2013-47257-P (http://www.mineco.gob.es/portal/site/mineco/)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)
dc.rights © 2020 Ana I. Núñez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject.other Sistema immunològic
dc.subject.other Mosquits
dc.subject.other Febre de la Vall del Rift
dc.subject.other Virus
dc.title Alteration in the Culex pipiens transcriptome reveals diverse mechanisms of the mosquito immune system implicated upon Rift Valley fever phlebovirus exposure
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pntd.0008870
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/AGL2013-47257-P
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics

In collaboration with Compliant to Partaking