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Functional characterization of the cell division gene cluster of the wall-less bacterium mycoplasma genitalium

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dc.contributor.author Martínez-Torró, Carlos
dc.contributor.author Torres-Puig, Sergi
dc.contributor.author Marcos-Silva, Marina
dc.contributor.author Huguet-Ramón, Marta
dc.contributor.author Muñoz-Navarro, Carmen
dc.contributor.author Lluch-Senar, Maria 1982-
dc.contributor.author Serrano Pubull, Luis, 1982-
dc.contributor.author Querol, Enrique
dc.contributor.author Piñol, Jaume
dc.contributor.author Pich, Oscar Q.
dc.date.accessioned 2022-01-19T12:29:46Z
dc.date.available 2022-01-19T12:29:46Z
dc.date.issued 2021
dc.identifier.citation Martínez-Torró C, Torres-Puig S, Marcos-Silva M, Huguet-Ramón M, Muñoz-Navarro C, Lluch-Senar M et al. Functional characterization of the cell division gene cluster of the wall-less bacterium mycoplasma genitalium. Front Microbiol. 2021 Sep 13;12:695572. [epub ahead of print]. DOI: 10.3389/fmicb.2021.695572
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10230/52262
dc.description Data de publicació electrònica: 13-09-2021
dc.description.abstract It is well-established that FtsZ drives peptidoglycan synthesis at the division site in walled bacteria. However, the function and conservation of FtsZ in wall-less prokaryotes such as mycoplasmas are less clear. In the genome-reduced bacterium Mycoplasma genitalium, the cell division gene cluster is limited to four genes: mraZ, mraW, MG_223, and ftsZ. In a previous study, we demonstrated that ftsZ was dispensable for growth of M. genitalium under laboratory culture conditions. Herein, we show that the entire cell division gene cluster of M. genitalium is non-essential for growth in vitro. Our analyses indicate that loss of the mraZ gene alone is more detrimental for growth of M. genitalium than deletion of ftsZ or the entire cell division gene cluster. Transcriptional analysis revealed a marked upregulation of ftsZ in the mraZ mutant. Stable isotope labeling by amino acids in cell culture (SILAC)-based proteomics confirmed the overexpression of FtsZ in MraZ-deprived cells. Of note, we found that ftsZ expression was upregulated in non-adherent cells of M. genitalium, which arise spontaneously at relatively high rates. Single cell analysis using fluorescent markers showed that FtsZ localization varied throughout the cell cycle of M. genitalium in a coordinated manner with the chromosome and the terminal organelle (TMO). In addition, our results indicate a possible role for the RNA methyltransferase MraW in the regulation of FtsZ expression at the post-transcriptional level. Altogether, this study provides an extensive characterization of the cell division gene cluster of M. genitalium and demonstrates the existence of regulatory elements controlling FtsZ expression at the temporal and spatial level in mycoplasmas.
dc.description.sponsorship This work was supported by the grant BIO2017-84166-R from the Ministerio de Ciencia, Innovación y Universidades
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Frontiers Media
dc.rights © 2021 Carlos Martínez-Torró et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.subject.other Genètica
dc.subject.other Bacteris
dc.subject.other Micoplasmes
dc.subject.other Mycoplasma genitalium
dc.title Functional characterization of the cell division gene cluster of the wall-less bacterium mycoplasma genitalium
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.3389/fmicb.2021.695572
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BIO2017-84166-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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