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MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation

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dc.contributor.author Bisceglie, Lavinia
dc.contributor.author Hopp, Ann-Katrin
dc.contributor.author Gunasekera, Kapila
dc.contributor.author Wright, Roni H.G.
dc.contributor.author Le Dily, François
dc.contributor.author Vidal, Enrique
dc.contributor.author Dall'Agnese, Alessandra
dc.contributor.author Caputo, Luca
dc.contributor.author Nicoletti, Chiara
dc.contributor.author Puri, Pier Lorenzo
dc.contributor.author Beato, Miguel
dc.contributor.author Hottiger, Michael O.
dc.date.accessioned 2021-11-25T06:51:45Z
dc.date.available 2021-11-25T06:51:45Z
dc.date.issued 2021
dc.identifier.citation Bisceglie L, Hopp AK, Gunasekera K, Wright RH, Le Dily F, Vidal E, Dall'Agnese A, Caputo L, Nicoletti C, Puri PL, Beato M, Hottiger MO. MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation. iScience. 2021;24(5):102432. DOI: 10.1016/j.isci.2021.102432
dc.identifier.issn 2589-0042
dc.identifier.uri http://hdl.handle.net/10230/49054
dc.description.abstract While protein ADP-ribosylation was reported to regulate differentiation and dedifferentiation, it has so far not been studied during transdifferentiation. Here, we found that MyoD-induced transdifferentiation of fibroblasts to myoblasts promotes the expression of the ADP-ribosyltransferase ARTD1. Comprehensive analysis of the genome architecture by Hi-C and RNA-seq analysis during transdifferentiation indicated that ARTD1 locally contributed to A/B compartmentalization and coregulated a subset of MyoD target genes that were however not sufficient to alter transdifferentiation. Surprisingly, the expression of ARTD1 was accompanied by the continuous synthesis of nuclear ADP ribosylation that was neither dependent on the cell cycle nor induced by DNA damage. Conversely to the H2O2-induced ADP-ribosylation, the MyoD-dependent ADP-ribosylation was not associated to chromatin but rather localized to the nucleoplasm. Together, these data describe a MyoD-induced nucleoplasmic ADP-ribosylation that is observed particularly during transdifferentiation and thus potentially expands the plethora of cellular processes associated with ADP-ribosylation.
dc.description.sponsorship Research in Beato's lab is supported by funds from the European Research Council under the European Union's Seventh Framework Program (FP7/2007-2013)/ERC Synergy grant agreement 609989 (4DGenome), from the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013-2017’ and Plan Nacional (SAF2016-75006-P), as well as support of the CERCA Program/Generalitat de Catalunya. L.B was supported by the Forschungskredit 2019 of the University of Zurich and K.G by a grant of the Oncosuisse (Nr. KFS-3740-08-2015-R). ADP-ribosylation research in the laboratory of MOH is funded by the Kanton of Zurich and the Swiss National Science Foundation, Switzerland (grant 31003A_176177).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof iScience. 2021;24(5):102432
dc.rights © 2021 The Author(s).This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.title MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.isci.2021.102432
dc.subject.keyword Biological sciences
dc.subject.keyword Cell biology
dc.subject.keyword Developmental biology
dc.subject.keyword Molecular biology
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/609989
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-75006-P
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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