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NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake

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dc.contributor.author Alari-Pahissa, Elisenda
dc.contributor.author Ataya Fernández, Michelle, 1993-
dc.contributor.author Moraitis, Ilias
dc.contributor.author Campos Ruiz, Miriam
dc.contributor.author Altadill, Mireia
dc.contributor.author Muntasell i Castellví, Aura, 1972-
dc.contributor.author Moles, Anna
dc.contributor.author López-Botet, Miguel
dc.date.accessioned 2021-10-20T06:29:23Z
dc.date.available 2021-10-20T06:29:23Z
dc.date.issued 2021
dc.identifier.citation Alari-Pahissa E, Ataya M, Moraitis I, Campos-Ruiz M, Altadill M, Muntasell A, Moles A, López-Botet M. NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake. PLoS Pathog. 2021;17(8):e1009868. DOI: 10.1371/journal.ppat.1009868
dc.identifier.issn 1553-7366
dc.identifier.uri http://hdl.handle.net/10230/48713
dc.description.abstract Epstein Barr virus (EBV) causes a highly prevalent and lifelong infection contributing to the development of some malignancies. In addition to the key role played by T cells in controlling this pathogen, NK cells mediate cytotoxicity and IFNγ production in response to EBV-infected B cells in lytic cycle, both directly and through antibody (Ab)-dependent activation. We recently described that EBV-specific Ab-dependent NK cell interaction with viral particles (VP) bound to B cells triggered degranulation and TNFα secretion but not B cell lysis nor IFNγ production. In this report we show that NK cell activation under these conditions reduced B cell transformation by EBV. NK cells eliminated VP from the surface of B cells through a specific and active process which required tyrosine kinase activation, actin polymerization and Ca2+, being independent of proteolysis and perforin. VP were displayed at the NK cell surface before being internalized and partially shuttled to early endosomes and lysosomes. VP transfer was encompassed by a trogocytosis process including the EBV receptor CD21, together with CD19 and CD20. Our study reveals a novel facet of the antibody-dependent NK cell mediated response to this viral infection.
dc.description.sponsorship The work was supported by grants SAF2016-80363-C2-1-R (MINECO/FEDER/UE), awarded to MLB by Ministerio de Economía y Competitividad and PID2019-110609RB-C21/AEI/ 10.13039/501100011033 awarded to MLB by Agencia Estatal de Investigación. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS Pathog. 2021;17(8):e1009868
dc.rights © 2021 Alari-Pahissa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.ppat.1009868
dc.subject.keyword NK cells
dc.subject.keyword B cells
dc.subject.keyword Cell staining
dc.subject.keyword Epstein-Barr virus
dc.subject.keyword Cell degranulation
dc.subject.keyword Cloning
dc.subject.keyword Cytotoxicity
dc.subject.keyword Monocytes
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SAF2016-80363-C2-1-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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