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Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling

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dc.contributor.author Hong, Shin-Young
dc.contributor.author Sun, Bin
dc.contributor.author Straub, Daniel
dc.contributor.author Blaakmeer, Anko
dc.contributor.author Mineri, Lorenzo
dc.contributor.author Koch, Jonas
dc.contributor.author Brinch-Pedersen, Henrik
dc.contributor.author Holme, Inger B.
dc.contributor.author Burow, Meike
dc.contributor.author Lyngs Jørgensen, Hans Jørgen
dc.contributor.author Albà Soler, Mar
dc.contributor.author Wenkel, Stephan
dc.date.accessioned 2021-03-24T06:57:56Z
dc.date.available 2021-03-24T06:57:56Z
dc.date.issued 2020
dc.identifier.citation Hong SY, Sun B, Straub D, Blaakmeer A, Mineri L, Koch J, Brinch-Pedersen H, Holme IB, Burow M, Lyngs Jørgensen HJ, Albà MM, Wenkel S. Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling. Proc Natl Acad Sci U S A. 2020; 117(42):26197-205. DOI: 10.1073/pnas.2005198117
dc.identifier.issn 0027-8424
dc.identifier.uri http://hdl.handle.net/10230/46911
dc.description.abstract MicroProteins are small, often single-domain proteins that are sequence-related to larger, often multidomain proteins. Here, we used a combination of comparative genomics and heterologous synthetic misexpression to isolate functional cereal microProtein regulators. Our approach identified LITTLE NINJA (LNJ), a microProtein that acts as a modulator of jasmonic acid (JA) signaling. Ectopic expression of LNJ in Arabidopsis resulted in stunted plants that resembled the decuple JAZ (jazD) mutant. In fact, comparing the transcriptomes of transgenic LNJ overexpressor plants and jazD revealed a large overlap of deregulated genes, suggesting that ectopic LNJ expression altered JA signaling. Transgenic Brachypodium plants with elevated LNJ expression levels showed deregulation of JA signaling as well and displayed reduced growth and enhanced production of side shoots (tiller). This tillering effect was transferable between grass species, and overexpression of LNJ in barley and rice caused similar traits. We used a clustered regularly interspaced short palindromic repeats (CRISPR) approach and created a LNJ-like protein in Arabidopsis by deleting parts of the coding sentence of the AFP2 gene that encodes a NINJA-domain protein. These afp2-crispr mutants were also stunted in size and resembled jazD Thus, similar genome-engineering approaches can be exploited as a future tool to create LNJ proteins and produce cereals with altered architectures.
dc.description.sponsorship Work in the laboratory was funded by the European Research Council (ERC-StG miPDesign, 336295), the Independent Research Fund Denmark (DFF-FNU, Adaptogenomics), the NovoNordisk Foundation (NNF18OC0034226), and start-up funding of Copenhagen Plant Science Centre through the University of Copenhagen. We thank the anonymous reviewers from a previous submission, Hans Thordal-Christensen, Gregg Howe, and Michael Broberg Palmgren for critically reading and improving our manuscript.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher National Academy of Sciences
dc.relation.ispartof Proc Natl Acad Sci U S A. 2020; 117(42):26197-205
dc.rights © 2020 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Heterologous microProtein expression identifies LITTLE NINJA, a dominant regulator of jasmonic acid signaling
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1073/pnas.2005198117
dc.subject.keyword Branching
dc.subject.keyword Jasmonic acid
dc.subject.keyword microProteins
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/336295
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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