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6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans

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dc.contributor.author Offenburger, Sarah-Lena
dc.contributor.author Gartner, Anton
dc.date.accessioned 2019-12-13T08:33:01Z
dc.date.available 2019-12-13T08:33:01Z
dc.date.issued 2018
dc.identifier.citation Offenburger SL, Gartner A. 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans. Bio Protoc. 2018; 8(18). pii: e3025. DOI 10.21769/BioProtoc.3025
dc.identifier.issn 2331-8325
dc.identifier.uri http://hdl.handle.net/10230/43158
dc.description.abstract The nematode Caenorhabditis elegans is a powerful genetic model that can be used to investigate neuronal death. Research using C. elegans has been crucial to characterize cell death programmes that are conserved in mammals. Many neuronal signaling components, such as those mediating dopaminergic neurotransmission, are preserved as well. Dopaminergic neurons are progressively lost in Parkinson's disease and an important risk factor to develop this disease appears to be oxidative stress, the increased occurrence of highly reactive oxygen species. Oxidative stress-induced dopaminergic neurodegeneration is mimicked in animal models by treatment with 6-hydroxydopamine (6-OHDA), a dopamine analog, which is specifically taken up into dopaminergic neurons. After exposing C. elegans to 6-OHDA, the loss of fluorescently labeled dopaminergic neurons can be easily monitored. An organisms' sensitivity to oxidative stress is thought to be influenced by basal levels of intrinsic oxidative stress and the ability to counteract oxidative stress and oxidative stress-induced damage. The C. elegans '6-OHDA model' led to the discovery of novel genes that are required to protect dopaminergic neurons and it has helped to determine the effects of conserved cell death and cell engulfment pathways in dopaminergic neurodegeneration. Here, we describe a simple protocol that allows for the easy detection of dopaminergic neuron loss after 6-OHDA treatment in C. elegans.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Bio-Protocol
dc.relation.ispartof Bio Protoc. 2018; 8(18). pii: e3025
dc.rights © 2018 The Authors; exclusive licensee Bio-protocol LLC.
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.title 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.21769/BioProtoc.3025
dc.subject.keyword 6-OHDA
dc.subject.keyword 6-hydroxydopamine
dc.subject.keyword C. elegans
dc.subject.keyword Caenorhabditis elegans
dc.subject.keyword Dopaminergic neurodegeneration
dc.subject.keyword Intoxication
dc.subject.keyword Oxidative stress assay
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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