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Extensive post-transcriptional buffering of gene expression in the response to severe oxidative stress in baker's yeast

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dc.contributor.author Blevins, William R.
dc.contributor.author Tavella, Teresa
dc.contributor.author Moro, Simone G.
dc.contributor.author Blasco Moreno, Bernat, 1986-
dc.contributor.author Closa-Mosquera, Adrià
dc.contributor.author Díez Antón, Juana, 1962-
dc.contributor.author Carey, Lucas
dc.contributor.author Albà Soler, Mar
dc.date.accessioned 2019-09-10T14:10:13Z
dc.date.available 2019-09-10T14:10:13Z
dc.date.issued 2019
dc.identifier.citation Blevins WR, Tavella T, Moro SG, Blasco-Moreno B, Closa-Mosquera A, Díez J, Carey LB, Albà MM. Extensive post-transcriptional buffering of gene expression in the response to severe oxidative stress in baker's yeast. Sci Rep. 2019; 9(1):11005. DOI 10.1038/s41598-019-47424-w
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10230/42262
dc.description.abstract Cells responds to diverse stimuli by changing the levels of specific effector proteins. These changes are usually examined using high throughput RNA sequencing data (RNA-Seq); transcriptional regulation is generally assumed to directly influence protein abundances. However, the correlation between RNA-Seq and proteomics data is in general quite limited owing to differences in protein stability and translational regulation. Here we perform RNA-Seq, ribosome profiling and proteomics analyses in baker's yeast cells grown in rich media and oxidative stress conditions to examine gene expression regulation at various levels. With the exception of a small set of genes involved in the maintenance of the redox state, which are regulated at the transcriptional level, modulation of protein expression is largely driven by changes in the relative ribosome density across conditions. The majority of shifts in mRNA abundance are compensated by changes in the opposite direction in the number of translating ribosomes and are predicted to result in no net change at the protein level. We also identify a subset of mRNAs which is likely to undergo specific translational repression during stress and which includes cell cycle control genes. The study suggests that post-transcriptional buffering of gene expression may be more common than previously anticipated.
dc.description.sponsorship The work was funded by grants BFU2015–65235-P, BFU2015-68351-P and BFU2016-80039-R, from Ministerio de Economía e Innovación (Spanish Government) - FEDER (EU), and from grant PT17/0009/0014 from Instituto de Salud Carlos III – FEDER. We also received funding from the “Maria de Maeztu” Programme for Units of Excellence in R&D (MDM-2014-0370) and from Agència de Gestió d’Ajuts Universitaris i de Recerca Generalitat de Catalunya (AGAUR), grant number 2014SGR1121, 2014SGR0974, 2017SGR01020 and, predoctoral fellowship (FI) to W.B. We also acknowledge support from the EU Erasmus Programme to T.T.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Nature Research
dc.relation.ispartof Sci Rep. 2019; 9(1):11005
dc.rights © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Extensive post-transcriptional buffering of gene expression in the response to severe oxidative stress in baker's yeast
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1038/s41598-019-47424-w
dc.subject.keyword Genome informatics
dc.subject.keyword Transcriptomics
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015–65235-P
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68351-P
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2016-80039-R
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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