Welcome to the UPF Digital Repository

In vivo force application reveals a fast tissue softening and external friction increase during early embryogenesis

Show simple item record

dc.contributor.author D'Angelo, Arturo
dc.contributor.author Dierkes, Kai
dc.contributor.author Carolis, Carlo
dc.contributor.author Salbreux, Guillaume
dc.contributor.author Solon, Jérôme
dc.date.accessioned 2019-07-30T07:42:24Z
dc.date.available 2019-07-30T07:42:24Z
dc.date.issued 2019
dc.identifier.citation D'Angelo A, Dierkes K, Carolis C, Salbreux G, Solon J. In vivo force application reveals a fast tissue softening and external friction increase during early embryogenesis. Curr Biol. 2019 May 6;29(9):1564-71. DOI: 10.1016/j.cub.2019.04.010
dc.identifier.issn 0960-9822
dc.identifier.uri http://hdl.handle.net/10230/42198
dc.description.abstract During development, cell-generated forces induce tissue-scale deformations to shape the organism [1,2]. The pattern and extent of these deformations depend not solely on the temporal and spatial profile of the generated force fields but also on the mechanical properties of the tissues that the forces act on. It is thus conceivable that, much like the cell-generated forces, the mechanical properties of tissues are modulated during development in order to drive morphogenesis toward specific developmental endpoints. Although many approaches have recently emerged to assess effective mechanical parameters of tissues [3-8], they could not quantitatively relate spatially localized force induction to tissue-scale deformations in vivo. Here, we present a method that overcomes this limitation. Our approach is based on the application of controlled forces on a single microparticle embedded in an individual cell of an embryo. Combining measurements of bead displacement with the analysis of induced deformation fields in a continuum mechanics framework, we quantify material properties of the tissue and follow their changes over time. In particular, we uncover a rapid change in tissue response occurring during Drosophila cellularization, resulting from a softening of the blastoderm and an increase of external friction. We find that the microtubule cytoskeleton is a major contributor to epithelial mechanics at this stage. We identify developmentally controlled modulations in perivitelline spacing that can account for the changes in friction. Overall, our method allows for the measurement of key mechanical parameters governing tissue-scale deformations and flows occurring during morphogenesis.
dc.description.sponsorship The research leading to these results has received funding from the Spanish Ministry of Economy and Competitiveness (MEIC) to the EMBL partnership, Plan Nacional, BFU2010-16546 and BFU2015-68754, and “Centro de Excelencia Severo Ochoa 2013–2017,” SEV-2012-0208. We acknowledge the support of the CERCA Programme/Generalitat de Catalunya. G.S. is supported by the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001317), UK Medical Research Council (FC001317), and Wellcome Trust (FC001317)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Current Biology. 2019 May 6;29(9):1564-71
dc.rights © 2019 Arturo D'Angelo et al. Published by Elsevier Ltd. This is an open access article under a CC BY license
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.other Embriologia
dc.subject.other Genètica
dc.title In vivo force application reveals a fast tissue softening and external friction increase during early embryogenesis
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.cub.2019.04.010
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2010-16546
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68754
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account


Compliant to Partaking