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Functional network analysis reveals the relevance of SKIIP in the regulation of alternative splicing by p38 SAPK

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dc.contributor.author Carbonell Núñez, Caterina, 1987-
dc.contributor.author Ulsamer, Arnau
dc.contributor.author Vivori, Claudia, 1989-
dc.contributor.author Papasaikas, Panagiotis
dc.contributor.author Böttcher, René
dc.contributor.author Joaquin i Caudet, Manel
dc.contributor.author Miñana Gómez, Belén
dc.contributor.author Tejedor Vaquero, Juan Ramón, 1984-
dc.contributor.author Nadal Clanchet, Eulàlia de
dc.contributor.author Valcárcel, J. (Juan)
dc.contributor.author Posas Garriga, Francesc
dc.date.accessioned 2019-06-20T06:36:27Z
dc.date.available 2019-06-20T06:36:27Z
dc.date.issued 2019
dc.identifier.citation Carbonell C, Ulsamer A, Vivori C, Papasaikas P, Böttcher R, Joaquin M, Miñana B, Tejedor JR, de Nadal E, Valcárcel J, Posas F. Functional network analysis reveals the relevance of SKIIP in the regulation of alternative splicing by p38 SAPK. Cell Rep. 2019; 27(3):847-859.e6. DOI 10.1016/j.celrep.2019.03.060
dc.identifier.issn 2211-1247
dc.identifier.uri http://hdl.handle.net/10230/41848
dc.description.abstract Alternative splicing is a prevalent mechanism of gene regulation that is modulated in response to a wide range of extracellular stimuli. Stress-activated protein kinases (SAPKs) play a key role in controlling several steps of mRNA biogenesis. Here, we show that osmostress has an impact on the regulation of alternative splicing (AS), which is partly mediated through the action of p38 SAPK. Splicing network analysis revealed a functional connection between p38 and the spliceosome component SKIIP, whose depletion abolished a significant fraction of p38-mediated AS changes. Importantly, p38 interacted with and directly phosphorylated SKIIP, thereby altering its activity. SKIIP phosphorylation regulated AS of GADD45α, the upstream activator of the p38 pathway, uncovering a negative feedback loop involving AS regulation. Our data reveal mechanisms and targets of SAPK function in stress adaptation through the regulation of AS.
dc.description.sponsorship This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (BFU2015-64437-P and FEDER, PGC2018-094136-B-I00 and FEDER, BFU2014-52125-REDT, and BFU2014-51672-REDC to F.P.; BFU2017-85152-P and FEDER to E.d.N.; BFU2014-005153 to J.V.), the Catalan Government (2017 SGR 799), the European Research Council (AdvG 670146 to J.V.), the Fundación Botín and the Banco Santander through its Santander Universities Global Division (to F.P. and J.V.), the Unidad de Excelencia Maria de Maeztu (MDM-2014-0370), and the Centre of Excellence Severo Ochoa. F.P. is a recipient of an ICREA Acadèmia (Generalitat de Catalunya).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Cell Rep. 2019; 27(3):847-859.e6
dc.rights © Elsevier This is the published version of an article http://dx.doi.org/10.1016/j.celrep.2019.03.060 that appeared in the journal Cell reports. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: https://www.elsevier.com/about/our-business/policies/open-access-licenses/elsevier-user-license
dc.rights.uri http://www.elsevier.com/open-access/userlicense/1.0/
dc.title Functional network analysis reveals the relevance of SKIIP in the regulation of alternative splicing by p38 SAPK
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.celrep.2019.03.060
dc.subject.keyword Cell signaling
dc.subject.keyword p38 SAPK
dc.subject.keyword Alternative splicing
dc.subject.keyword Stress responses
dc.subject.keyword GADD45
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-64437-P
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-52125-REDT
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-51672-REDC
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BFU2017-85152-P
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-005153
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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