Lamin B1 mapping reveals the existence of dynamic and functional euchromatin lamin B1 domains

Pascual-Reguant, Laura; Blanco, Enrique; Galan Martínez, Silvia, 1992-; Le Dily, François; Cuartero, Yasmina; Serra Bardenys, Gemma, 1992-; Di Carlo, Valerio; Iturbide Martínez de Albéniz, Ane, 1989-; Cebrià i Costa, Joan Pau, 1989-; Nonell Mazelon, Lara, 1972-; García de Herreros, Antonio; Di Croce, Luciano; Marti-Renom, Marc A.; Peiró Sales, Sandra

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dc.contributor.author	Pascual-Reguant, Laura
dc.contributor.author	Blanco, Enrique
dc.contributor.author	Galan Martínez, Silvia, 1992-
dc.contributor.author	Le Dily, François
dc.contributor.author	Cuartero, Yasmina
dc.contributor.author	Serra Bardenys, Gemma, 1992-
dc.contributor.author	Di Carlo, Valerio
dc.contributor.author	Iturbide Martínez de Albéniz, Ane, 1989-
dc.contributor.author	Cebrià i Costa, Joan Pau, 1989-
dc.contributor.author	Nonell Mazelon, Lara, 1972-
dc.contributor.author	García de Herreros, Antonio
dc.contributor.author	Di Croce, Luciano
dc.contributor.author	Marti-Renom, Marc A.
dc.contributor.author	Peiró Sales, Sandra
dc.date.accessioned	2018-10-31T13:20:28Z
dc.date.available	2018-10-31T13:20:28Z
dc.date.issued	2018
dc.identifier.citation	Pascual-Reguant L, Blanco E, Galan S, Le Dily F, Cuartero Y, Serra-Bardenys G et al. Lamin B1 mapping reveals the existence of dynamic and functional euchromatin lamin B1 domains. Nat Commun. 2018; 9(1):3420. DOI 10.1038/s41467-018-05912-z
dc.identifier.issn	2041-1723
dc.identifier.uri	http://hdl.handle.net/10230/35687
dc.description.abstract	Lamins (A/C and B) are major constituents of the nuclear lamina (NL). Structurally conserved lamina-associated domains (LADs) are formed by genomic regions that contact the NL. Lamins are also found in the nucleoplasm, with a yet unknown function. Here we map the genome-wide localization of lamin B1 in an euchromatin-enriched fraction of the mouse genome and follow its dynamics during the epithelial-to-mesenchymal transition (EMT). Lamin B1 associates with actively expressed and open euchromatin regions, forming dynamic euchromatin lamin B1-associated domains (eLADs) of about 0.3 Mb. Hi-C data link eLADs to the 3D organization of the mouse genome during EMT and correlate lamin B1 enrichment at topologically associating domain (TAD) borders with increased border strength. Having reduced levels of lamin B1 alters the EMT transcriptional signature and compromises the acquisition of mesenchymal traits. Thus, during EMT, the process of genome reorganization in mouse involves dynamic changes in eLADs
dc.description.sponsorship	This work was supported by grants from the Instituto de Salud Carlos III (ISCIII) FIS/FEDER (PI15/00396; CPII14/0006), Ministerio de Economía y Competitividad (MINECO) (SAF2013-40922-R1; FPU14/0407; BFU2016-75008-P), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional-FEDER (SAF2016-76461-R), Generalitat de Catalunya (2014 SGR 32), Fundació FERO, Fundació La Marató TV3, and La Caixa Foundation. We also thank the Advanced Light Microscopy Unit at the CRG for their assistance and the Cellex Foundation for providing research facilities and equipment. M.A.M.-R. acknowledges funding from the European Research Council under the 7th Framework Program (FP7/2010-2015, ERC grant agreement 609989), the European Union’s Horizon 2020 research and innovation program (agreement 676556), the Spanish Ministry of Economy and Competitiveness (BFU2013-47736-P), and the Centro de Excelencia Severo Ochoa 2013-2017 (SEV-2012-0208) to the CRG
dc.format.mimetype	application/pdf
dc.language.iso	eng
dc.publisher	Nature Publishing Group
dc.relation.ispartof	Nature Communications. 2018;9(1):3420
dc.rights	© The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.title	Lamin B1 mapping reveals the existence of dynamic and functional euchromatin lamin B1 domains
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	http://dx.doi.org/10.1038/s41467-018-05912-z
dc.subject.keyword	DNA
dc.subject.keyword	Nuclear organization
dc.relation.projectID	info:eu-repo/grantAgreement/ES/1PE/SAF2013-40922-R1
dc.relation.projectID	info:eu-repo/grantAgreement/ES/1PE/BFU2016-75008-P
dc.relation.projectID	info:eu-repo/grantAgreement/ES/1PE/BFU2013-47736-P
dc.relation.projectID	info:eu-repo/grantAgreement/EC/FP7/609989
dc.relation.projectID	info:eu-repo/grantAgreement/EC/H2020/676556
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.type.version	info:eu-repo/semantics/publishedVersion