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Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus

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dc.contributor.author de Jaime Soguero, Anchel
dc.contributor.author Aulicino, Francesco, 1987-
dc.contributor.author Ertaylan, Gokhan
dc.contributor.author Griego, Anna
dc.contributor.author Cerrato, Aniello
dc.contributor.author Tallam, Aravind
dc.contributor.author del Sol, Antonio
dc.contributor.author Cosma, Maria Pia, 1970-
dc.contributor.author Lluis Viñas, Frederic
dc.date.accessioned 2018-07-04T07:56:38Z
dc.date.available 2018-07-04T07:56:38Z
dc.date.issued 2017
dc.identifier.citation De Jaime-Soguero A, Aulicino F, Ertaylan G, Griego A, Cerrato A, Tallam A et al. Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus. PLoS Genet. 2017 Mar 27;13(3):e1006682. DOI: 10.1371/journal.pgen.1006682
dc.identifier.issn 1553-7390
dc.identifier.uri http://hdl.handle.net/10230/35028
dc.description.abstract Understanding the mechanisms regulating cell cycle, proliferation and potency of pluripotent stem cells guarantees their safe use in the clinic. Embryonic stem cells (ESCs) present a fast cell cycle with a short G1 phase. This is due to the lack of expression of cell cycle inhibitors, which ultimately determines naïve pluripotency by holding back differentiation. The canonical Wnt/β-catenin pathway controls mESC pluripotency via the Wnt-effector Tcf3. However, if the activity of the Wnt/β-catenin controls the cell cycle of mESCs remains unknown. Here we show that the Wnt-effector Tcf1 is recruited to and triggers transcription of the Ink4/Arf tumor suppressor locus. Thereby, the activation of the Wnt pathway, a known mitogenic pathway in somatic tissues, restores G1 phase and drastically reduces proliferation of mESCs without perturbing pluripotency. Tcf1, but not Tcf3, is recruited to a palindromic motif enriched in the promoter of cell cycle repressor genes, such as p15Ink4b, p16Ink4a and p19Arf, which mediate the Wnt-dependent anti-proliferative effect in mESCs. Consistently, ablation of β-catenin or Tcf1 expression impairs Wnt-dependent cell cycle regulation. All together, here we showed that Wnt signaling controls mESC pluripotency and proliferation through non-overlapping functions of distinct Tcf factors.
dc.description.sponsorship We are grateful for the support from ERC grant (242630-RERE) (MPC), the Ministerio de Economia y Competitividad y FEDER (SAF2011-28580, and BFU2014-54717-P, BFU2015-71984-ERC to MPC), an AGAUR grant from Secretaria d´Universitats i Investigació del Departament d´Economia i Coneixement de la Generalitat de Catalunya (2014SGR1137 to MPC), Ministerio de Ciencia e Innovación FPI (to FA), the European Union's Horizon 2020 research and innovation programme under grant agreement CellViewer No 686637 (to MPC), the Spanish Ministry of Economy and Competitiveness, Centro de Excelencia Severo Ochoa 2013-2017, the CERCA Programme/Generalitat de Catalunya (to MPC); KU Leuven starting grant (STG) and KU Leuven C1 funds (C14/16/078) (to FL), AFR Postdoctoral Grant from the Luxembourg National Research Fund (FNR); ANEMO project N. 4001584/PDR 2012-1 and Dutch Province of Limburg (to GE), Short Term Mobility Award, CNR (to AC).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLOS Genetics. 2017 Mar 27;13(3):e1006682
dc.rights © 2017 De Jaime-Soguero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1371/journal.pgen.1006682
dc.subject.keyword Cell cycle inhibitors
dc.subject.keyword Cell division
dc.subject.keyword Cell proliferation
dc.subject.keyword Cell staining
dc.subject.keyword Gene expression regulation
dc.subject.keyword G1 phase
dc.subject.keyword Cloning
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/242630
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/SAF2011-28580
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2014-54717-P
dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-71984-ERC
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/686637
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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