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dc.contributor.author | Ecker, Simone |
dc.contributor.author | Merkel, Angelika |
dc.contributor.author | BLUEPRINT Consortium |
dc.contributor.author | Paul, Dirk S. |
dc.date.accessioned | 2017-05-26T07:52:38Z |
dc.date.available | 2017-05-26T07:52:38Z |
dc.date.issued | 2017 |
dc.identifier.citation | Ecker S, Chen L, Pancaldi V, Bagger FO, Fernández JM, Carrillo de Santa Pau E et al. Genome-wide analysis of differential transcriptional and epigenetic variability across human immune cell types. Genome Biology. 2017;18:18. DOI: 10.1186/s13059-017-1156-8 |
dc.identifier.issn | 1474-760X |
dc.identifier.uri | http://hdl.handle.net/10230/32168 |
dc.description.abstract | Background: A healthy immune system requires immune cells that adapt rapidly to environmental challenges. This phenotypic plasticity can be mediated by transcriptional and epigenetic variability. Results: We apply a novel analytical approach to measure and compare transcriptional and epigenetic variability genome-wide across CD14+CD16− monocytes, CD66b+CD16+ neutrophils, and CD4+CD45RA+ naïve T cells from the same 125 healthy individuals. We discover substantially increased variability in neutrophils compared to monocytes and T cells. In neutrophils, genes with hypervariable expression are found to be implicated in key immune pathways and are associated with cellular properties and environmental exposure. We also observe increased sex-specific gene expression differences in neutrophils. Neutrophil-specific DNA methylation hypervariable sites are enriched at dynamic chromatin regions and active enhancers. Conclusions: Our data highlight the importance of transcriptional and epigenetic variability for the key role of neutrophils as the first responders to inflammatory stimuli. We provide a resource to enable further functional studies into the plasticity of immune cells, which can be accessed from: http://blueprint-dev.bioinfo.cnio.es/WP10/hypervariability. |
dc.description.sponsorship | This work is predominantly funded by the EU-FP7 Project BLUEPRINT (HEALTH-F5-2011-282510). |
dc.format.mimetype | application/pdf |
dc.language.iso | eng |
dc.publisher | BioMed Central |
dc.relation.ispartof | Genome Biology. 2017;18:18 |
dc.rights | © Ecker E, et al. 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ |
dc.title | Genome-wide analysis of differential transcriptional and epigenetic variability across human immune cell types |
dc.type | info:eu-repo/semantics/article |
dc.identifier.doi | http://dx.doi.org/10.1186/s13059-017-1156-8 |
dc.subject.keyword | Differential variability |
dc.subject.keyword | Phenotypic plasticity |
dc.subject.keyword | Heterogeneity |
dc.subject.keyword | Immune cells |
dc.subject.keyword | Monocytes |
dc.subject.keyword | Neutrophils |
dc.subject.keyword | T cells |
dc.subject.keyword | Gene expression |
dc.subject.keyword | DNA methylation |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/282510 |
dc.rights.accessRights | info:eu-repo/semantics/openAccess |
dc.type.version | info:eu-repo/semantics/publishedVersion |