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Molecular Interaction and Cellular Location of RecA and CheW Proteins in Salmonella enterica during SOS Response and Their Implication in Swarming

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dc.contributor.author Irazoki, Oihane
dc.contributor.author Aranda, Jesús
dc.contributor.author Zimmermann, Timo
dc.contributor.author Campoy, Susana
dc.contributor.author Barbé, Jordi
dc.date.accessioned 2017-01-18T08:39:13Z
dc.date.available 2017-01-18T08:39:13Z
dc.date.issued 2016
dc.identifier.citation Irazoki O, Aranda J, Zimmermann T, Campoy S and Barbé J. Molecular Interaction and Cellular Location of RecA and CheW Proteins in Salmonella enterica during SOS Response and Their Implication in Swarming. Frontiers in Microbiology. 2016; 7: 1560. DOI: 10.3389/fmicb.2016.01560
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10230/27922
dc.description.abstract In addition to its role in DNA damage repair and recombination, the RecA protein, through its interaction with CheW, is involved in swarming motility, a form of flagella-dependent movement across surfaces. In order to better understand how SOS response modulates swarming, in this work the location of RecA and CheW proteins within the swarming cells has been studied by using super-resolution microscopy. Further, and after in silico docking studies, the specific RecA and CheW regions associated with the RecA-CheW interaction have also been confirmed by site-directed mutagenesis and immunoprecipitation techniques. Our results point out that the CheW distribution changes, from the cell poles to foci distributed in a helical pattern along the cell axis when SOS response is activated or RecA protein is overexpressed. In this situation, the CheW presents the same subcellular location as that of RecA, pointing out that the previously described RecA storage structures may be modulators of swarming motility. Data reported herein not only confirmed that the RecA-CheW pair is essential for swarming motility but it is directly involved in the CheW distribution change associated to SOS response activation. A model explaining not only the mechanism by which DNA damage modulates swarming but also how both the lack and the excess of RecA protein impair this motility is proposed.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Frontiers
dc.relation.ispartof Frontiers in Microbiology. 2016; 7: 1560
dc.rights © 2016 Irazoki, Aranda, Zimmermann, Campoy and Barbé. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.uri https://creativecommons.org/licenses/by/4.0/
dc.title Molecular Interaction and Cellular Location of RecA and CheW Proteins in Salmonella enterica during SOS Response and Their Implication in Swarming
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.3389/fmicb.2016.01560
dc.subject.keyword SOS response
dc.subject.keyword Swarming
dc.subject.keyword Chemoreceptor polar arrays
dc.subject.keyword Chemosensory cluster assembly
dc.subject.keyword RecA
dc.subject.keyword CheW
dc.subject.keyword 3D-STED
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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