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Genome sequencing and secondary metabolism of the postharvest pathogen Penicillium griseofulvum

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dc.contributor.author Banani, Houda
dc.contributor.author Marcet Houben, Marina
dc.contributor.author Ballester, Ana Rosa
dc.contributor.author Abbruscato, Pamela
dc.contributor.author González Candelas, Luis
dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
dc.contributor.author Spadaro, Davide
dc.date.accessioned 2016-06-03T16:28:10Z
dc.date.available 2016-06-03T16:28:10Z
dc.date.issued 2016
dc.identifier.citation Banani H, Marcet-Houben M, Ballester AR, Abbruscato P, González-Candelas L, Gabaldón T, Spadaro D. Genome sequencing and secondary metabolism of the postharvest pathogen Penicillium griseofulvum. BMC Genomics. 2016; 17(1): 19. DOI 10.1186/s12864-015-2347-x
dc.identifier.issn 1471-2164
dc.identifier.uri http://hdl.handle.net/10230/26823
dc.description.abstract BACKGROUND: Penicillium griseofulvum is associated in stored apples with blue mould, the most important postharvest disease of pome fruit. This pathogen can simultaneously produce both detrimental and beneficial secondary metabolites (SM). In order to gain insight into SM synthesis in P. griseofulvum in vitro and during disease development on apple, we sequenced the genome of P. griseofulvum strain PG3 and analysed important SM clusters. RESULTS: PG3 genome sequence (29.3 Mb) shows that P. griseofulvum branched off after the divergence of P. oxalicum but before the divergence of P. chrysogenum. Genome-wide analysis of P. griseofulvum revealed putative gene clusters for patulin, griseofulvin and roquefortine C biosynthesis. Furthermore, we quantified the SM production in vitro and on apples during the course of infection. The expression kinetics of key genes of SM produced in infected apple were examined. We found additional SM clusters, including those potentially responsible for the synthesis of penicillin, yanuthone D, cyclopiazonic acid and we predicted a cluster putatively responsible for the synthesis of chanoclavine I. CONCLUSIONS: These findings provide relevant information to understand the molecular basis of SM biosynthesis in P. griseofulvum, to allow further research directed to the overexpression or blocking the synthesis of specific SM.
dc.description.sponsorship Work at the University of Torino was partially supported by the LIFE financial instrument of the European Union (Contract LIFE13 ENV/HR/000580). TG group research was partially funded by a grant from the Spanish Ministry of Economy and Competitiveness (BIO2012-37161), a grant from the Qatar National Research Fund (NPRP5-298-3-086), and a grant from the European Research Council (Grant Agreement ERC-2012-StG-310325). Work at LGC lab was partially supported by a grant from the Spanish Ministry of Economy and Innovation (AGL2011-30519-C03-01) and by the Generalitat Valenciana, Spain (PROMETEOII/2014/027). ARB is grateful to CSIC and the European Social Fund for her postdoctoral contract JAE-Doc
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartof BMC Genomics. 2016; 17(1): 19
dc.relation.ispartof Penicillium griseofulvum
dc.relation.ispartof Genome sequencing
dc.relation.ispartof Secondary metabolites
dc.relation.ispartof Postharvest disease
dc.relation.ispartof Blue mold
dc.relation.ispartof Patulin
dc.relation.ispartof Roquefortine C
dc.relation.ispartof Griseofulvin
dc.rights © 2015 Banani et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.other Genomes
dc.title Genome sequencing and secondary metabolism of the postharvest pathogen Penicillium griseofulvum
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1186/s12864-015-2347-x
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2012-37161
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/310325
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


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