Welcome to the UPF Digital Repository

ADF/cofilin regulates secretory cargo sorting at the TGN via the Ca2+ ATPase SPCA1

Show simple item record

dc.contributor.author Blume, Julia von
dc.contributor.author Alleaume, Anne-Marie
dc.contributor.author Cantero Recasens, Gerard, 1984-
dc.contributor.author Curwin, Amy
dc.contributor.author Carreras Sureda, Amado, 1986-
dc.contributor.author Zimmermann, Timo
dc.contributor.author Galen, Josse van
dc.contributor.author Wakana, Yuichi
dc.contributor.author Valverde, M. A. (Miguel Ángel), 1963-
dc.contributor.author Malhotra, Vivek
dc.date.accessioned 2015-12-17T14:19:19Z
dc.date.available 2015-12-17T14:19:19Z
dc.date.issued 2011
dc.identifier.citation von Blume J, Alleaume AM, Cantero-Recasens G, Curwin A, Carreras-Sureda A, Zimmermann T et al. ADF/cofilin regulates secretory cargo sorting at the TGN via the Ca2+ ATPase SPCA1. Developmental Cell. 2011. 20(5): 652-662. DOI 10.1016/j.devcel.2011.03.014
dc.identifier.issn 1534-5807
dc.identifier.uri http://hdl.handle.net/10230/25458
dc.description.abstract Actin-severing proteins ADF/cofilin are required for the sorting of secretory cargo at the trans-Golgi network (TGN) in mammalian cells. How do these cytoplasmic proteins interact with the cargoes in the lumen of the TGN? Put simply, how are these two sets of proteins connected across the TGN membrane? Mass spectrometry of cofilin1 immunoprecipitated from HeLa cells revealed the presence of actin and the Ca(2+) ATPase SPCA1. Moreover, cofilin1 was localized to the TGN and bound to SPCA1 via dynamic actin. SPCA1 knockdown, like ADF/cofilin1 knockdown, inhibited Ca(2+) uptake into the TGN and caused missorting of secretory cargo. These defects were rescued by the overexpression of the TGN-localized SPCA1. We propose that ADF/cofilin-dependent severing of actin filaments exposes and promotes the activation of SPCA1, which pumps Ca(2+) into the lumen of the TGN for the sorting of the class of secretory cargo that binds Ca(2+).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Developmental Cell. 2011. 20(5): 652-662
dc.rights © Elsevier This is the published version of an article http://dx.doi.org/10.1016/j.devcel.2011.03.014 that appeared in the journal Developmental Cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-license
dc.subject.other Calci -- Metabolisme
dc.subject.other Cèl·lules HeLa
dc.title ADF/cofilin regulates secretory cargo sorting at the TGN via the Ca2+ ATPase SPCA1
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.devcel.2011.03.014
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion


This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account


Compliant to Partaking