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A 12-gene expression signature is associated with aggressive histological in prostate cancer: SEC14L1 and TCEB1 genes are potential markers of progression. American journal of pathology

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dc.contributor.author Agell, Laia
dc.contributor.author Hernández, Silvia
dc.contributor.author Nonell Mazelón, Lara
dc.contributor.author Lorenzo Perez, Marta
dc.contributor.author Puigdecanet Riubugent, Eulàlia
dc.contributor.author Muga, Silvia de
dc.contributor.author Juanpere, Nuria
dc.contributor.author Bermudo, Raquel
dc.contributor.author Fernández, Pedro Luís
dc.contributor.author Lorente Garin, José Antonio
dc.contributor.author Serrano Figueras, Sergi
dc.contributor.author Lloreta Trull, Josep, 1958-
dc.date.accessioned 2015-12-07T14:00:40Z
dc.date.available 2015-12-07T14:00:40Z
dc.date.issued 2012
dc.identifier.citation Agell L, Hernández S, Nonell L, Lorenzo M, Puigdecanet E, de Muga S et al. A 12-gene expression signature is associated with aggressive histological in prostate cancer: SEC14L1 and TCEB1 genes are potential markers of progression. Am J Pathol. 2012;181(5):1585-94. DOI: 10.1016/j.ajpath.2012.08.005
dc.identifier.issn 0002-9440
dc.identifier.uri http://hdl.handle.net/10230/25342
dc.description.abstract The main challenge for clinical management of prostate cancer is to distinguish tumors that will progress faster and will show a higher tendency to recur from the more indolent ones. We have compared expression profiles of 18 prostate cancer samples (seven with a Gleason score of 6, eight with a Gleason score of 7, and three with a Gleason score of ≥8) and five nonneoplastic prostate samples, using the Affymetrix Human Array GeneChip Exon 1.0 ST. Microarray analysis revealed 99 genes showing statistically significant differences among tumors with Gleason scores of 6, 7, and ≥8. In addition, mRNA expression of 29 selected genes was analyzed by real-time quantitative RT-PCR with microfluidic cards in an extended series of 30 prostate tumors. Of the 29 genes, 18 (62%) were independently confirmed in the extended series by quantitative RT-PCR: 14 were up-regulated and 4 were down-regulated in tumors with a higher Gleason score. Twelve of these genes were differentially expressed in tumors with a Gleason score of 6 to 7 versus ≥8. Finally, IHC validation of the protein levels of two genes from the 12-gene signature (SEC14L1 and TCEB1) showed strong protein expression levels of both genes, which were statistically associated with a high combined Gleason score, advanced stage, and prostate-specific antigen progression. This set of genes may contribute to a better understanding of the molecular basis of prostate cancer. TCEB1 and SELC14L1 are good candidate markers for predicting prognosis and progression of prostate cancer.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof American journal of pathology. 2012;181(5):1585-94
dc.rights © Elsevier. This is the published version of an article http://dx.doi.org/10.1016/j.ajpath.2012.08.005 that appeared in the journal American journal of pathology. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-license
dc.subject.other Pròstata
dc.subject.other Transcripció genètica Regulació
dc.subject.other Proteïnes portadores
dc.title A 12-gene expression signature is associated with aggressive histological in prostate cancer: SEC14L1 and TCEB1 genes are potential markers of progression. American journal of pathology
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.ajpath.2012.08.005
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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