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Complete DNA sequence of Kuraishia capsulata illustrates novel genomic features among budding yeasts (Saccharomycotina)

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dc.contributor.author Morales, Lucia
dc.contributor.author Noel, Benjamin
dc.contributor.author Porcel, Betina
dc.contributor.author Marcet Houben, Marina
dc.contributor.author Hullo, Marie-Françoise
dc.contributor.author Sacerdot, Christine
dc.contributor.author Tekaia, Fredj
dc.contributor.author Leh-Louis, Véronique
dc.contributor.author Despons, Laurence
dc.contributor.author Khanna, Varun
dc.contributor.author Aury, Jean-Marc
dc.contributor.author Barbe, Valérie
dc.contributor.author Coloux, Arnaud
dc.contributor.author Labadie, Karine
dc.contributor.author Pelletier, Eric
dc.contributor.author Souciet, Jean-Luc
dc.contributor.author Boekhout, Teun
dc.contributor.author Gabaldón Estevan, Juan Antonio, 1973-
dc.contributor.author Wincker, Patrick
dc.contributor.author Dujon, Bernard
dc.date.accessioned 2014-05-14T07:34:10Z
dc.date.available 2014-05-14T07:34:10Z
dc.date.issued 2013
dc.identifier.citation Morales L, Noel B, Porcel B, Marcet-Houben M, Hullo MF, Sacerdot C et al. Complete DNA sequence of Kuraishia capsulata illustrates novel genomic features among budding yeasts (Saccharomycotina). Genome Biol Evol. 2013; 5(12): 2524-2539. DOI 10.1093/gbe/evt201
dc.identifier.issn 1759-6653
dc.identifier.uri http://hdl.handle.net/10230/22481
dc.description.abstract The numerous yeast genome sequences presently available provide a rich source of information for functional as well as evolutionary genomics but unequally cover the large phylogenetic diversity of extant yeasts. We present here the complete sequence of the nuclear genome of the haploid-type strain of Kuraishia capsulata (CBS1993(T)), a nitrate-assimilating Saccharomycetales of uncertain taxonomy, isolated from tunnels of insect larvae underneath coniferous barks and characterized by its copious production of extracellular polysaccharides. The sequence is composed of seven scaffolds, one per chromosome, totaling 11.4 Mb and containing 6,029 protein-coding genes, 13.5% of which being interrupted by introns. This GC-rich yeast genome (45.7%) appears phylogenetically related with the few other nitrate-assimilating yeasts sequenced so far, Ogataea polymorpha, O. parapolymorpha, and Dekkera bruxellensis, with which it shares a very reduced number of tRNA genes, a novel tRNA sparing strategy, and a common nitrate assimilation cluster, three specific features to this group of yeasts. Centromeres were recognized in GC-poor troughs of each scaffold. The strain bears MAT alpha genes at a single MAT locus and presents a significant degree of conservation with Saccharomyces cerevisiae genes, suggesting that it can perform sexual cycles in nature, although genes involved in meiosis were not all recognized. The complete absence of conservation of synteny between K. capsulata and any other yeast genome described so far, including the three other nitrate-assimilating species, validates the interest of this species for long-range evolutionary genomic studies among Saccharomycotina yeasts.
dc.description.sponsorship Sequencing was supported by CEA/Genoscope and performed/nusing HPC resources from GENCI-TGCC (Grants 2012076389 and 2013036389). Data analysis and experimental aspects of the work were supported in part 1) by contract DYGEVO from ANR (2011SVSE6) to B.D., 2) by the/nSpanish ministry of Economy and Competitiveness(BIO2012-37161) and the European Research Council (Grant Agreement n ERC-2012-StG-310325) to T.G., and 3) by the/nQatar National Research Fund grant (NPRP 5-298-3-086) to T.B. and T.G.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Oxford University Press
dc.rights © 2013 Lucia Morales et al. This is an Open Acces article distributed under a Creative Commons Attribution Non-commercial License
dc.rights.uri http://creativecommons.org/licenses/by/3.0/
dc.subject.other Genòmica
dc.subject.other Llevats
dc.title Complete DNA sequence of Kuraishia capsulata illustrates novel genomic features among budding yeasts (Saccharomycotina)
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1093/gbe/evt201
dc.relation.projectID info:eu-repo/grantAgreement/EC/ERC/310325
dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BIO2012-37161
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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