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Multiple non-collinear TF-map alignments of promoter regions

Mostra el registre parcial de l'element Blanco, Enrique Guigó Serra, Roderic Messeguer, Xavier
dc.contributor.other Universitat Pompeu Fabra 2011-10-26T11:07:52Z 2011-10-26T11:07:52Z 2007
dc.identifier.citation Blanco E, Guigó R, Messeguer X. Multiple non-collinear TF-map alignments of promoter regions. BMC Bioinformatics. 2007; 8: 138. DOI 10.1186/1471-2105-8-138
dc.identifier.issn 1471-2105
dc.description.abstract Background: The analysis of the promoter sequence of genes with similar expression patterns is/na basic tool to annotate common regulatory elements. Multiple sequence alignments are on the/nbasis of most comparative approaches. The characterization of regulatory regions from coexpressed/ngenes at the sequence level, however, does not yield satisfactory results in many/noccasions as promoter regions of genes sharing similar expression programs often do not show/nnucleotide sequence conservation./nResults: In a recent approach to circumvent this limitation, we proposed to align the maps of/npredicted transcription factors (referred as TF-maps) instead of the nucleotide sequence of two/nrelated promoters, taking into account the label of the corresponding factor and the position in the/nprimary sequence. We have now extended the basic algorithm to permit multiple promoter/ncomparisons using the progressive alignment paradigm. In addition, non-collinear conservation/nblocks might now be identified in the resulting alignments. We have optimized the parameters of/nthe algorithm in a small, but well-characterized collection of human-mouse-chicken-zebrafish/northologous gene promoters./nConclusion: Results in this dataset indicate that TF-map alignments are able to detect high-level/nregulatory conservation at the promoter and the 3'UTR gene regions, which cannot be detected/nby the typical sequence alignments. Three particular examples are introduced here to illustrate the/npower of the multiple TF-map alignments to characterize conserved regulatory elements in/nabsence of sequence similarity. We consider this kind of approach can be extremely useful in the/nfuture to annotate potential transcription factor binding sites on sets of co-regulated genes from/nhigh-throughput expression experiments.
dc.language.iso eng
dc.publisher BioMed Central
dc.rights © 2007 Blanco et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This article is also available from:
dc.subject.other Factors de transcripció
dc.subject.other Genètica -- Tècnica
dc.title Multiple non-collinear TF-map alignments of promoter regions
dc.type info:eu-repo/semantics/article
dc.subject.keyword Promoter regions
dc.subject.keyword Nucleotide sequence
dc.subject.keyword TF-map alignment
dc.subject.keyword Non-collinear conservation blocks
dc.subject.keyword 3'UTR
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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