Cutibacterium acnes-derived extracellular vesicles: characterization and modulation of the skin immune response

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• dc.contributor.author Pol Cros, Maria
• dc.date.accessioned 2021-07-20T08:38:51Z
• dc.date.issued 2021-07
• dc.description Tutor: María José Fábrega Fernández
• dc.description.abstract The human microbiome plays an essential role in maintaining the host health. One of the most common bacteria present in the skin microbiota is Cutibacterium acnes (C.acnes) which is known for being involved in the homeostasis of the skin, but in some other cases, it can act as an opportunistic pathogen contributing to some skin alterations like acne vulgaris. Recently, some authors have claimed that different strains of C. acnes have different degrees of association with acne vulgaris. For instance, it has been shown that C. acnes subtype H1 was present in patients who had never developed acne vulgaris before. Therefore, a protective role has been hypothesized for this phylotype. In addition, C. acnes extracellular vesicles (EVs) have been reported to play an important role in the inflammatory profile of skin cells. Nevertheless, their protein, lipid and nucleic acid content have not been fully characterized yet. In the present project, EVs have been isolated from different C. acnes strains (A1, H1 and KPA) and their cytotoxicity has been tested with an immortalized keratinocyte cell line (HaCaT) and an immortalized sebocytes cell line (SZ95). In parallel, the immuno-modulation role of these EVs has been studied using a 2D skin inflammatory model, which mimics the in vivo skin layers considering both the epithelial and the immune system. On the other hand, it is important to know the content of the EVs to determine if they could have a probiotic behavior themselves or, otherwise, be an appropriate drug delivery vehicle. RNA sequencing and a proteomic analysis are going to be conducted in order to elucidate it.ca
• dc.embargo.liftdate 2022-01-01
• dc.format.mimetype application/pdf*
• dc.identifier.uri http://hdl.handle.net/10230/48246
• dc.language.iso engca
• dc.rights © Tots els drets reservatsca
• dc.rights.accessRights info:eu-repo/semantics/embargoedAccessca
• dc.subject.keyword Microbiome
• dc.subject.keyword Extracellular vesicles (EV)
• dc.subject.keyword Cutibacterium acnes
• dc.subject.keyword Skin inflammation
• dc.subject.keyword Acne vulgaris
• dc.title Cutibacterium acnes-derived extracellular vesicles: characterization and modulation of the skin immune responseca
• dc.type info:eu-repo/semantics/bachelorThesisca