La relació entre les proteïnes long-non-coding i l'Alzheimer

Abstract

The aim of this study is to find the relation between some of the long-non-coding proteines derived from the brain and the Alzheimer’s disease which had a significant difference between a healthy brain and one which had Alzheimer in the preclinical state. It could be essential for the diagnosis of this illness since the extracellular vesicles could be biomarkers of it and they can be extracted from the blood Consequenly, with only a blood analysis information from the brain could be obtained. To do it some samples of post mortem brains that didn’t have the disease, which are called control samples, had been extracted as well as samples of post mortem brains which did have Alzheimer in the preclinical state of the disease. Out of this samples, there were extracted the extracellular vesicles and, later, the proteic part of it was divided into smaller units called peptides (like a part of a protein). After that, they were characterised using mass spectometry. Finally, using an informatic programme, the data collected the proteins which were derived from the non-coding regions of the estracellular vesicles of the brain. The analysis of the ones that had a significant difference between the control and the preclinical state of the Alzheimer’s disease could be done in order to find the relation of this proteins with this neurodegenerative disease. The results of the experiment turned out to be that, among all the proeteins which were studied, there were some of them which were more expressed in the preclinical state of the Alzheimer’s disease and their function was related to the development of the illness. In contrast, some were more expressed in the control group and had a function which was related with the prevention of the Alzheimer’s disease. Notwithstanding, there were some proteins which it is known that they have a relation with this disease although it is not known how wheras some of the proteins did not even had a relation with Alzheimer. Moreover, there were proteins that, despite having more in the brain than in the brain was affected by the disease, its function was related to the development of the Alzheimer's disease. Because of that this project is the beginning of a new research for which, in the same project, two hypotheses are already proposed.