Kuderna, Lukas, 1989-Lizano González, Esther, 1974-Julià, EvaGómez Garrido, JèssicaSerres Armero, Aitor, 1992-Kuhlwilm, MartinAlandes, Regina AntoniAlvarez-Estape, MarinaJuan, DavidSimon, HeathAlioto, TylerGut, MartaGut, Ivo GlynneSchierup, Mikkel H.Fornas Carreño, OscarMarquès i Bonet, Tomàs, 1975-2019-03-112019-03-112019Kuderna LFK, Lizano E, Julià E, Gomez-Garrido J, Serres-Armero A, Kuhlwilm M et al. Selective single molecule sequencing and assembly of a human Y chromosome of African origin. Nat Commun. 2019;10(1):4. DOI: 10.1038/s41467-018-07885-52041-1723http://hdl.handle.net/10230/36784Mammalian Y chromosomes are often neglected from genomic analysis. Due to their inherent assembly difficulties, high repeat content, and large ampliconic regions, only a handful of species have their Y chromosome properly characterized. To date, just a single human reference quality Y chromosome, of European ancestry, is available due to a lack of accessible methodology. To facilitate the assembly of such complicated genomic territory, we developed a novel strategy to sequence native, unamplified flow sorted DNA on a MinION nanopore sequencing device. Our approach yields a highly continuous assembly of the first human Y chromosome of African origin. It constitutes a significant improvement over comparable previous methods, increasing continuity by more than 800%. Sequencing native DNA also allows to take advantage of the nanopore signal data to detect epigenetic modifications in situ. This approach is in theory generalizable to any species simplifying the assembly of extremely large and repetitive genomes.application/pdfeng© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41467-018-07885-5Comparative genomicsGenome assembly algorithmsGenomicsinfo:eu-repo/semantics/openAccess