Pervouchine, Dmitri D.Djebali, SarahBreschi, Alessandra, 1988-Prieto Barja, Pablo, 1986-Lagarde, JulienBussotti, Giovanni, 1983-Monlong, JeanNotredame, CedricGuigó Serra, RodericGingeras, Thomas R.2015-11-162015-11-162015Pervouchine DD, Djebali S, Breschi A, Davis CA, Barja PP, Dobin A et al. Enhanced transcriptome maps from multiple mouse tissues reveal evolutionary constraint in gene expression. Nature communications. 2015;6:5903. DOI: 10.1038/ncomms69032041-1723http://hdl.handle.net/10230/25109Mice have been a long-standing model for human biology and disease. Here we characterize, by RNA sequencing, the transcriptional profiles of a large and heterogeneous collection of mouse tissues, augmenting the mouse transcriptome with thousands of novel transcript candidates. Comparison with transcriptome profiles in human cell lines reveals substantial conservation of transcriptional programmes, and uncovers a distinct class of genes with levels of expression that have been constrained early in vertebrate evolution. This core set of genes captures a substantial fraction of the transcriptional output of mammalian cells, and participates in basic functional and structural housekeeping processes common to all cell types. Perturbation of these constrained genes is associated with significant phenotypes including embryonic lethality and cancer. Evolutionary constraint in gene expression levels is not reflected in the conservation of the genomic sequences, but is associated with conserved epigenetic marking, as well as with characteristic post-transcriptional regulatory programme, in which sub-cellular localization and alternative splicing play comparatively large roles.application/pdfeng© 2015 Macmillan Publishers Limited. All rights reserved. This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.Ratolins -- GenèticaRegulació genèticainfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/ncomms6903info:eu-repo/semantics/openAccess