Borràs, EvaSabidó Aguadé, Eduard, 1981-2018-06-142017Borràs E, Sabidó E. What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry. Proteomics. 2017 Sep;17(17-18):1700180. DOI: 10.1002/pmic.2017001801615-9853http://hdl.handle.net/10230/34896Targeted proteomics has gained significant popularity in mass spectrometry-based protein quantification as a method to detect proteins of interest with high sensitivity, quantitative accuracy and reproducibility. However, with the emergence of a wide variety of targeted proteomics methods, some of them with high-throughput capabilities, it is easy to overlook the essence of each method and to determine what makes each of them a targeted proteomics method. In this viewpoint, we revisit the main targeted proteomics methods and classify them in four categories differentiating those methods that perform targeted data acquisition from targeted data analysis, and those methods that are based on peptide ion data (MS1 targeted methods) from those that rely on the peptide fragments (MS2 targeted methods).application/pdfengThis is the peer reviewed version of the following article: Borràs E, Sabidó E. What is targeted proteomics? A concise revision of targeted acquisition and targeted data analysis in mass spectrometry. Proteomics. 2017 Sep;17(17-18):1700180, which has been published in final form at http://dx.doi.org/10.1002/pmic.201700180. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/pmic.201700180Data independent acquisitionParallel reaction monitoringMass spectrometryTargeted proteomicsSelected reaction monitoringinfo:eu-repo/semantics/openAccess