Savol, Andrej J.Wang, Peggy I.Jeon, YesuColognori, DavidYildirim, EdaPinter, Stefan F.Payer, BernhardLee, Jeannie T.Sadreyev, Ruslan Ilyasovich2018-07-132018-07-132017Savol AJ, Wang PI, Jeon Y, Colognori D, Yildirim E, Pinter SF et al. Genome-wide identification of autosomal genes with allelic imbalance of chromatin state. PLoS One. 2017 Aug 10;12(8):e0182568. DOI: 10.1371/journal.pone.01825681932-6203http://hdl.handle.net/10230/35149In mammals, monoallelic gene expression can result from X-chromosome inactivation, genomic imprinting, and random monoallelic expression (RMAE). Epigenetic regulation of RMAE is not fully understood. Here we analyze allelic imbalance in chromatin state of autosomal genes using ChIP-seq in a clonal cell line. We identify approximately 3.7% of autosomal genes that show significant differences between chromatin states of two alleles. Allelic regulation is represented among several functional gene categories including histones, chromatin modifiers, and multiple early developmental regulators. Most cases of allelic skew are produced by quantitative differences between two allelic chromatic states that belong to the same gross type (active, silent, or bivalent). Combinations of allelic states of different types are possible but less frequent. When different chromatin marks are skewed on the same gene, their skew is coordinated as a result of quantitative relationships between these marks on each individual allele. Finally, combination of allele-specific densities of chromatin marks is a quantitative predictor of allelic skew in gene expression.application/pdfeng© 2017 Savol et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.info:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0182568Gene expressionChromatinHistonesInbred strainsDNA transcriptioninfo:eu-repo/semantics/openAccess