Laguillo Diego, Alejandra, 1991-Kiewisz, RobertMartí-Gómez, CarlosBaum, DanielMüller-Reichert, ThomasVernos, Isabelle, 1959-2023-02-242023-02-242023Laguillo-Diego A, Kiewisz R, Martí-Gómez C, Baum D, Müller-Reichert T, Vernos I. MCRS1 modulates the heterogeneity of microtubule minus-end morphologies in mitotic spindles. Mol Biol Cell. 2023 Jan 1;34(1):ar1. DOI: 10.1091/mbc.E22-08-0306-T1059-1524http://hdl.handle.net/10230/55902Faithful chromosome segregation requires the assembly of a bipolar spindle, consisting of two antiparallel microtubule (MT) arrays having most of their minus ends focused at the spindle poles and their plus ends overlapping in the spindle midzone. Spindle assembly, chromosome alignment, and segregation require highly dynamic MTs. The plus ends of MTs have been extensively investigated but their minus-end structure remains poorly characterized. Here, we used large-scale electron tomography to study the morphology of the MT minus ends in three dimensionally reconstructed metaphase spindles in HeLa cells. In contrast to the homogeneous open morphology of the MT plus ends at the kinetochores, we found that MT minus ends are heterogeneous, showing either open or closed morphologies. Silencing the minus end-specific stabilizer, MCRS1 increased the proportion of open MT minus ends. Altogether, these data suggest a correlation between the morphology and the dynamic state of the MT ends. Taking this heterogeneity of the MT minus-end morphologies into account, our work indicates an unsynchronized behavior of MTs at the spindle poles, thus laying the groundwork for further studies on the complexity of MT dynamics regulation.application/pdfeng© 2023 Laguillo-Diego, Kiewisz, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution 4.0 International Creative Commons CC-BY 4.0 License (https://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.1091/mbc.E22-08-0306-Tinfo:eu-repo/semantics/openAccess