Pizzagalli, Diego UlisseSintes, JordiFernández González, Santiago2019-07-162019-07-162018Pizzagalli DU, Farsakoglu Y, Palomino-Segura M, Palladino E, Sintes J, Marangoni F. et al. Leukocyte Tracking Database, a collection of immune cell tracks from intravital 2-photon microscopy videos. Sci Data. 2018 Jul 17;5:180129. DOI: 10.1038/sdata.2018.1292052-4463http://hdl.handle.net/10230/42000Recent advances in intravital video microscopy have allowed the visualization of leukocyte behavior in vivo, revealing unprecedented spatiotemporal dynamics of immune cell interaction. However, state-of-the-art software and methods for automatically measuring cell migration exhibit limitations in tracking the position of leukocytes over time. Challenges arise both from the complex migration patterns of these cells and from the experimental artifacts introduced during image acquisition. Additionally, the development of novel tracking tools is hampered by the lack of a sound ground truth for algorithm validation and benchmarking. Therefore, the objective of this work was to create a database, namely LTDB, with a significant number of manually tracked leukocytes. Broad experimental conditions, sites of imaging, types of immune cells and challenging case studies were included to foster the development of robust computer vision techniques for imaging-based immunological research. Lastly, LTDB represents a step towards the unravelling of biological mechanisms by video data mining in systems biology.application/pdfengOpen Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadatafiles made available in this articleLeucòcitsCèl·lules -- Interaccióinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/sdata.2018.129info:eu-repo/semantics/openAccess