Percy, Andrew J.Espadas, GuadalupeSabidó Aguadé, Eduard, 1981-Borchers, Christoph H.2024-10-222024-10-222015Percy AJ, Tamura-Wells J, Albar JP, Aloria K, Amirkhani A, Araujo GD, et al. Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment. EuPA Open Proteom. 2015 Sep;8:6-15. DOI: 10.1016/j.euprot.2015.06.0012212-9685http://hdl.handle.net/10230/68293The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.application/pdfeng© 2015 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).info:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.euprot.2015.06.001AccuracyHuman plasmaQuality controlQuantitative proteomicsReproducibilityWorkflow validationinfo:eu-repo/semantics/openAccess