Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs

Bohórquez, José AlejandroDefaus, SiraRosell, RosaPérez-Simó, MartaAlberch, MònicaGladue, Douglas P.Borca, Manuel V.Andreu Martínez, DavidGanges, Lliliane2021-11-122021-11-122021Bohórquez JA, Defaus S, Rosell R, Pérez-Simó M, Alberch M, Gladue DP, Borca MV, Andreu D, Ganges L. Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigs. Viruses. 2021;13(10):1980. DOI: 10.3390/v131019801999-4915http://hdl.handle.net/10230/48960Classical swine fever virus (CSFV) causes a viral disease of high epidemiological and economical significance that affects domestic and wild swine. Control of the disease in endemic countries is based on live-attenuated vaccines (LAVs) that induce an early protective immune response against highly virulent CSFV strains. The main disadvantage of these currently available LAVs is the lack of serological techniques to differentiate between vaccinated and infected animals (DIVA concept). Here, we describe the development of the FlagDIVA test, a serological diagnostic tool allowing for the differentiation between animals vaccinated with the FlagT4G candidate and those infected with CSFV field strains. The FlagDIVA test is a direct ELISA based on a dendrimeric peptide construct displaying a conserved epitope of CSFV structural protein E2. Although FlagDIVA detected anti-CSFV anti-bodies in infected animals, it did not recognize the antibody response of FlagT4G-vaccinated animals. Therefore, the FlagDIVA test constitutes a valuable accessory DIVA tool in implementing vaccination with the FlagT4G candidate.application/pdfeng© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Development of a dendrimeric peptide-based approach for the differentiation of animals vaccinated with FlagT4G against classical swine fever from infected pigsinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/v13101980CSFV live-attenuated vaccineFlagT4G CSFV vaccine candidateSerological DIVA testinfo:eu-repo/semantics/openAccess