Gaspa, LauraGonzález Medina, Alberto, 1990-Hidalgo Hernando, ElenaAyté del Olmo, José2016-04-122016Gaspa L, González-Medina A, Hidalgo E, Ayté J. A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave. Cell cycle. 2016; 15(5): 720-729. DOI 10.1080/15384101.2016.11488391538-4101http://hdl.handle.net/10230/26073The Schizosaccharomyces pombe MBF complex activates the transcription of genes required for DNA synthesis and S phase. The MBF complex contains several proteins, including the core components Cdc10, Res1 and Res2, the co-repressor proteins Yox1 and Nrm1 and the co-activator Rep2. It has recently been shown how MBF is regulated when either the DNA damage or the DNA synthesis checkpoints are activated. However, how MBF is regulated in a normal unperturbed cell cycle is still not well understood. We have set up a genome-wide genomic screen searching for global regulators of MBF. We have crossed our knock-out collection library with a reporter strain that allows the measurement of MBF activity in live cells by flow cytometry. We confirm previously known regulators of MBF and show that COP9/signalosome and tRNA methyltransferases also regulate MBF activityapplication/pdfeng© Taylor & Francis. This is an electronic version of an article published in Gaspa L, González-Medina A, Hidalgo E, Ayté J. A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave. Cell cycle. 2016; 15(5): 720-729. DOI 10.1080/15384101.2016.1148839. Cell cycle is available online at: www.tandfonline.com http://www.tandfonline.com/doi/pdf/10.1080/15384101.2016.1148839.Schizosaccharomyces pombe -- MetabolismeADNinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/15384101.2016.1148839COP9Cdc22-YFPElongatorG1/S transitionMBF complexS. pombeinfo:eu-repo/semantics/openAccess