Dejea i Velardo, HèctorSchlepütz, Christian M.Méndez-Carmona, NataliaArnold, MariaGarcía Cañadilla, Patricia, 1985-Longnus, Sarah L.Stampanoni, MarcoBijnens, BartBonnin, Anne2025-10-202025-10-202022Dejea H, Schlepütz CM, Méndez-Carmona N, Arnold M, Garcia-Canadilla P, Longnus SL, et al. A tomographic microscopy-compatible Langendorff system for the dynamic structural characterization of the cardiac cycle. Front Cardiovasc Med. 2022 Dec 22;9:1023483. DOI: 10.3389/fcvm.2022.10234832297-055Xhttp://hdl.handle.net/10230/71556Introduction: Cardiac architecture has been extensively investigated ex vivo using a broad spectrum of imaging techniques. Nevertheless, the heart is a dynamic system and the structural mechanisms governing the cardiac cycle can only be unveiled when investigating it as such. Methods: This work presents the customization of an isolated, perfused heart system compatible with synchrotron-based X-ray phase contrast imaging (X-PCI). Results: Thanks to the capabilities of the developed setup, it was possible to visualize a beating isolated, perfused rat heart for the very first time in 4D at an unprecedented 2.75 μm pixel size (10.6 μm spatial resolution), and 1 ms temporal resolution. Discussion: The customized setup allows high-spatial resolution studies of heart architecture along the cardiac cycle and has thus the potential to serve as a tool for the characterization of the structural dynamics of the heart, including the effects of drugs and other substances able to modify the cardiac cycle.application/pdfeng© 2022 Dejea, Schlepütz, Méndez-Carmona, Arnold, Garcia-Canadilla, Longnus, Stampanoni, Bijnens and Bonnin. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.info:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fcvm.2022.1023483SynchrotronTomographic microscopyLangendorff4D imagingCardiac cycleinfo:eu-repo/semantics/openAccess