Olarte, Omar E.Licea-Rodriguez, JacobPalero, Jonathan A.Gualda, Emilio J.Artigas, DavidMayer, Jürgen, 1977-Swoger, JimSharpe, JamesRocha-Mendoza, IsraelRangel-Rojo, RaulLoza-Alvarez, Pablo2018-07-192018-07-192012Olarte OE, Licea-Rodriguez J, Palero JA, Gualda EJ, Artigas D, Mayer J et al. Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles. Biomed Opt Express. 2012 Jul 1;3(7):1492-505. DOI: 10.1364/BOE.3.0014922156-7085http://hdl.handle.net/10230/35196We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivoCaenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view.application/pdfeng© Oman E. Olarte et al 2012 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reservedFeixos de làserMicroscòpia de fluorescènciaMicroscòpiaProcessos multifotóinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1364/BOE.3.001492info:eu-repo/semantics/openAccess