Schellhas, LauraMonasso, Giulietta S.Felix, Janine FrédériqueJaddoe, Vincent W. V.Huang, PeiyuanFernández-Barrés, SílviaVrijheid, MartinePesce, GiancarloAnnesi-Maesano, IsabellaPage, Christian M.Brantsaeter, Anne LiseBekkhus, MonaHåberg, Siri E.London, Stephanie J.Munafò, Marcus R.Zuccolo, LuisaSharp, Gemma C.2024-06-192024-06-192023Schellhas L, Monasso GS, Felix JF, Jaddoe VW, Huang P, Fernández-Barrés S, et al. Maternal caffeine consumption during pregnancy and offspring cord blood DNA methylation: an epigenome-wide association study meta-analysis. Epigenomics. 2023 Nov;15(22):1179-93. DOI: 10.2217/epi-2023-02631750-1911http://hdl.handle.net/10230/60518Background: Prenatal caffeine exposure may influence offspring health via DNA methylation, but no large studies have tested this. Materials & methods: Epigenome-wide association studies and differentially methylated regions in cord blood (450k or EPIC Illumina arrays) were meta-analyzed across six European cohorts (n = 3725). Differential methylation related to self-reported caffeine intake (mg/day) from coffee, tea and cola was compared with assess whether caffeine is driving effects. Results: One CpG site (cg19370043, PRRX1) was associated with caffeine and another (cg14591243, STAG1) with cola intake. A total of 12-22 differentially methylated regions were detected with limited overlap across caffeinated beverages. Conclusion: We found little evidence to support an intrauterine effect of caffeine on offspring DNA methylation. Statistical power limitations may have impacted our findings.application/pdfeng© 2023 The Authors. This work is licensed under the Creative Commons Attribution 4.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.info:eu-repo/semantics/articlehttp://dx.doi.org/10.2217/epi-2023-0263DNA methylationPACE consortiumCaffeineEpigeneticsOffspring healthPregnancyinfo:eu-repo/semantics/openAccess