Orom, Ulf AnderssonDerrien, ThomasBeringer, MalteGumireddy, KiranmaiGardini, AlessandroBussotti, Giovanni, 1983-Lai, FanZytnicki, MatthiasNotredame, CedricHuang, QihongGuigó Serra, RodericShiekhattar, Ramin2016-01-072016-01-072010Orom UA, Derrien T, Beringer M, Gumireddy K, Gardini A, Bussotti G et al. Long noncoding RNAs with enhancer-like function in human cells. Cell. 2010; 143(1): 46-58. DOI 10.1016/j.cell.2010.09.0010092-8674http://hdl.handle.net/10230/25539While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE annotation of the human genome to characterize over a thousand long ncRNAs that are expressed in multiple cell lines. Unexpectedly, we found an enhancer-like function for a set of these long ncRNAs in human cell lines. Depletion of a number of ncRNAs led to decreased expression of their neighboring protein-coding genes, including the master regulator of hematopoiesis, SCL (also called TAL1), Snai1 and Snai2. Using heterologous transcription assays we demonstrated a requirement for the ncRNAs in activation of gene expression. These results reveal an unanticipated role for a class of long ncRNAs in activation of critical regulators of development and differentiation.application/pdfeng© Elsevier. This is the published version of an article http://dx.doi.org/10.1016/j.cell.2010.09.001 that appeared in the journal Cell. It is published in an Open Archive under an Elsevier user license. Details of this licence are available here: http://www.elsevier.com/about/open-access/open-access-policies/oa-license-policy/elsevier-user-licenseRNA missatgerFactors de transcripcióinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cell.2010.09.001info:eu-repo/semantics/openAccess